Titre:	Enhancing the antimicrobial activity of silver nanoparticles against pathogenic bacteria by using Pelargonium sidoides DC extract in microwave assisted green synthesis
Auteur(s):	Illanes Tormena, Renata Pascoal Santos, Mac-Kedson Medeiros Salviano Silva, Atailson Oliveira da Félix, Felipe Mourthé Chaker, Juliano Alexandre Freire, Daniel Oliveira Silva, Izabel Cristina Rodrigues da Moya, Sergio Enrique Sousa, Marcelo Henrique
metadata.dc.identifier.orcid:	http://orcid.org/0000-0003-3248-1286 http://orcid.org/0000-0001-6964-3002 http://orcid.org/0000-0001-7434-6491 http://orcid.org/0000-0003-1586-7961 http://orcid.org/0000-0002-1577-7862 http://orcid.org/0000-0002-6836-3583 http://orcid.org/0000-0002-7174-1960 http://orcid.org/0000-0003-1210-8329
metadata.dc.contributor.affiliation:	University of Brasilia, Green Nanotechnology Group University of Brasilia, Green Nanotechnology Group University of Brasilia, Green Nanotechnology Group University of Brasilia, Green Nanotechnology Group University of Brasília, Faculty of Ceilandia, Graduate Program in Health Sciences and Technologies University of Brasília, Faculty of Ceilandia, Graduate Program in Health Sciences and Technologies CIC biomaGUNE, Soft Matter Nanotechnology Laboratory University of Brasilia, Green Nanotechnology Group
Assunto::	Nanopartículas de prata Atividade antimicrobiana Síntese verde
Date de publication:	jui-2024
Editeur:	Royal Society of Chemistry
Référence bibliographique:	TORMENA, Renata Pascoal Illanes et al. Enhancing the antimicrobial activity of silver nanoparticles against pathogenic bacteria by using Pelargonium sidoides DC extract in microwave assisted green synthesis. RSC Advances, [S. l.], v. 14, 22035, 2024. DOI: https://doi.org/10.1039/D4RA04140B. Disponível em: https://pubs.rsc.org/en/content/articlehtml/2024/ra/d4ra04140b. Acesso em: 7 mai. 2026.
Abstract:	This study presents an optimized microwave-assisted method for the green synthesis of silver nanoparticles (AgNPs) using a root extract obtained from Pelargonium sidoides DC. The influence of temperature, reagent concentration, and irradiation time was systematically investigated to enhance synthesis yield. Characterization techniques including XRD, UV-vis, FTIR, XPS, and zetametry were employed to confirm the successful formation of nanoparticles with a metallic silver core (∼17 nm) functionalized with organic molecules derived from the plant extract. The cytotoxicity of AgNPs was assessed using a cell viability assay, while the Minimum Inhibitory Concentration (MIC) of nanoformulation against pathogenic bacteria, including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and the carbapenem-resistant Klebsiella pneumoniae (KPC), was determined using the Broth microdilution method. The nanoformulation synthesized with P. sidoides extract exhibited a dose-dependent response, demonstrating superior antimicrobial efficacy compared to the pure plant extract in most cases. The MIC values ranged from 0.85 to 17.1 μg mL−1, with particularly strong performance against the drug resistant KPC strain. The enhanced antimicrobial effect is attributed to the synergistic action of the metallic silver core and phytochemicals from P. sidoides on the surface of nanoparticles, which also contribute to notable colloidal stability of AgNPs at physiological pH levels.
metadata.dc.description.unidade:	Instituto de Química (IQ)
metadata.dc.description.ppg:	Programa de Pós-Graduação em Ciências e Tecnologias em Saúde
Licença::	This Open Access Article is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported Licence
DOI:	https://doi.org/10.1039/D4RA04140B
Collection(s) :	Artigos publicados em periódicos e afins

Tous les documents dans DSpace sont protégés par copyright, avec tous droits réservés.