Título :	Erythropoietin effects on cryopreserved/transplanted cat ovarian tissue : a comparison of two incubation methods
Autor :	Silva, Isabella Monteiro Gomes da Rodrigues, Aline de Queiroz Ribeiro, Rayane Brandão Aguiar, Beatriz Regina Lima de Marinho, Anne Elisabeth Schineider Pereira Souza, Elisa dos Anjos Mendes de Ferreira, Yasmin Barboza Azevedo, Victoria Caroline de Oliveira Oliveira, Daniela Mara de Báo, Sônia Nair Goulart, Jair Trapé Lucci, Carolina Madeira Paulini, Fernanda
metadata.dc.contributor.email:	mailto:fepaulini@unb.br
metadata.dc.identifier.orcid:	https://orcid.org/0000-0002-2832-5726 https://orcid.org/0000-0002-9873-3098 https://orcid.org/0000-0003-0737-1077 https://orcid.org/0000-0001-7763-0746 https://orcid.org/0000-0003-4490-9614
metadata.dc.contributor.affiliation:	University of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences University of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences University of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences University of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences University of Brasilia, Health Sciences Faculty, Department of Pharmacy University of Brasilia, Health Sciences Faculty, Department of Pharmacy University of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences University of Brasilia, Health Sciences Faculty, Department of Pharmacy University of Brasilia, Institute of Biological Sciences, Department of Genetics an d Morpholog University of Brasilia, Institute of Biological Sciences, Department of Cellular Biology University of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences University of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences University of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences
Assunto::	Germoplasma Folículos ovarianos Criopreservação de órgãos, tecidos, etc. Angiogênese Gato
Fecha de publicación :	27-feb-2024
Editorial :	Elsevier
Citación :	SILVA, Isabella M.G. et. al. Erythropoietin effects on cryopreserved/transplanted cat ovarian tissue: a comparison of two incubation methods. Cryobiology, v. 115, art. 104861, jun. 2024. DOI 10.1016/j.cryobiol.2024.104861. Disponível em: https://www.sciencedirect.com/science/article/pii/S0011224024000166?via%3Dihub. Acesso em: 17 mar. 2025.
Abstract:	Many feline species are currently threatened with extinction. Therefore, germplasm bank establishment has become imperative. However, cryoinjury and ischemia-reperfusion injury pose significant obstacles to both cryopreservation and xenotransplantation. In this regard, erythropoietin (Epo) represents a potential alternative strategy due to its properties. This study aimed to assess the incubation of domestic cat ovarian tissue in Epo, both before and after cryopreservation, and investigate its effectiveness in promoting revascularization following xenotransplantation. Sixteen ovaries from 8 healthy cats were sliced following elective bilateral ovariohysterectomy (OHE). Subsequently, 8 fragments measuring 3 mm³ each were obtained from the cortical region of each ovary. The fragments were allocated into 3 treatment groups: Cryo group, fragments were cryopreserved, thawed and immediately transplanted; Cryo + Epo group, fragments were first cryopreserved in nitrogen, thawed, incubated in Epo (100 IU) for 2h and transplanted; and the Epo + Cryo group, in which fragments were first incubated in Epo (100 IU) for 2h, cryopreserved, thawed and immediately transplanted. The fragments were then xenotransplanted into the dorsal subcutaneous region of ovariectomized female nude mice and retrieved at 7, 14, 21, and 28 days post-transplantation. The results indicated that Epo effectively enhanced follicular survival, preservation of viability, and tissue revascularization. The Epo + Cryo group displayed better revascularization rates on D14 and D21 post-transplantation and an increase in primordial and growing follicles on D28, the Cryo + Epo group exhibited significantly more follicles on D14 and D21, with fewer degenerated follicles.
metadata.dc.description.unidade:	Instituto de Ciências Biológicas (IB) Departamento de Ciências Fisiológicas (IB CFS) Departamento de Biologia Celular (IB CEL) Departamento de Genética e Morfologia (IB GEM) Faculdade de Ciências da Saúde (FS) Departamento de Farmácia (FS FAR)
Licença::	© 2024 Society for Cryobiology. Published by Elsevier Inc. All rights reserved.
DOI:	https://doi.org/10.1016/j.cryobiol.2024.104861
metadata.dc.relation.publisherversion:	https://www.sciencedirect.com/science/article/pii/S0011224024000166?via%3Dihub
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