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Universidade de Brasília
Use este identificador para citar ou linkar para este item: http://repositorio.unb.br/handle/10482/51957
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dc.contributor.authorSilva, Isabella Monteiro Gomes da-
dc.contributor.authorRodrigues, Aline de Queiroz-
dc.contributor.authorRibeiro, Rayane Brandão-
dc.contributor.authorAguiar, Beatriz Regina Lima de-
dc.contributor.authorMarinho, Anne Elisabeth Schineider Pereira-
dc.contributor.authorSouza, Elisa dos Anjos Mendes de-
dc.contributor.authorFerreira, Yasmin Barboza-
dc.contributor.authorAzevedo, Victoria Caroline de Oliveira-
dc.contributor.authorOliveira, Daniela Mara de-
dc.contributor.authorBáo, Sônia Nair-
dc.contributor.authorGoulart, Jair Trapé-
dc.contributor.authorLucci, Carolina Madeira-
dc.contributor.authorPaulini, Fernanda-
dc.date.accessioned2025-03-17T22:53:57Z-
dc.date.available2025-03-17T22:53:57Z-
dc.date.issued2024-02-27-
dc.identifier.citationSILVA, Isabella M.G. et. al. Erythropoietin effects on cryopreserved/transplanted cat ovarian tissue: a comparison of two incubation methods. Cryobiology, v. 115, art. 104861, jun. 2024. DOI 10.1016/j.cryobiol.2024.104861. Disponível em: https://www.sciencedirect.com/science/article/pii/S0011224024000166?via%3Dihub. Acesso em: 17 mar. 2025.pt_BR
dc.identifier.urihttp://repositorio.unb.br/handle/10482/51957-
dc.language.isoengpt_BR
dc.publisherElsevierpt_BR
dc.rightsAcesso Restritopt_BR
dc.titleErythropoietin effects on cryopreserved/transplanted cat ovarian tissue : a comparison of two incubation methodspt_BR
dc.typeArtigopt_BR
dc.subject.keywordGermoplasmapt_BR
dc.subject.keywordFolículos ovarianospt_BR
dc.subject.keywordCriopreservação de órgãos, tecidos, etc.pt_BR
dc.subject.keywordAngiogênesept_BR
dc.subject.keywordGatopt_BR
dc.rights.license© 2024 Society for Cryobiology. Published by Elsevier Inc. All rights reserved.pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.cryobiol.2024.104861pt_BR
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0011224024000166?via%3Dihubpt_BR
dc.description.abstract1Many feline species are currently threatened with extinction. Therefore, germplasm bank establishment has become imperative. However, cryoinjury and ischemia-reperfusion injury pose significant obstacles to both cryopreservation and xenotransplantation. In this regard, erythropoietin (Epo) represents a potential alternative strategy due to its properties. This study aimed to assess the incubation of domestic cat ovarian tissue in Epo, both before and after cryopreservation, and investigate its effectiveness in promoting revascularization following xenotransplantation. Sixteen ovaries from 8 healthy cats were sliced following elective bilateral ovariohysterectomy (OHE). Subsequently, 8 fragments measuring 3 mm³ each were obtained from the cortical region of each ovary. The fragments were allocated into 3 treatment groups: Cryo group, fragments were cryopreserved, thawed and immediately transplanted; Cryo + Epo group, fragments were first cryopreserved in nitrogen, thawed, incubated in Epo (100 IU) for 2h and transplanted; and the Epo + Cryo group, in which fragments were first incubated in Epo (100 IU) for 2h, cryopreserved, thawed and immediately transplanted. The fragments were then xenotransplanted into the dorsal subcutaneous region of ovariectomized female nude mice and retrieved at 7, 14, 21, and 28 days post-transplantation. The results indicated that Epo effectively enhanced follicular survival, preservation of viability, and tissue revascularization. The Epo + Cryo group displayed better revascularization rates on D14 and D21 post-transplantation and an increase in primordial and growing follicles on D28, the Cryo + Epo group exhibited significantly more follicles on D14 and D21, with fewer degenerated follicles.pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-2832-5726pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-9873-3098pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-0737-1077pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-7763-0746pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-4490-9614pt_BR
dc.contributor.emailmailto:fepaulini@unb.brpt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciencespt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciencespt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciencespt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciencespt_BR
dc.contributor.affiliationUniversity of Brasilia, Health Sciences Faculty, Department of Pharmacypt_BR
dc.contributor.affiliationUniversity of Brasilia, Health Sciences Faculty, Department of Pharmacypt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciencespt_BR
dc.contributor.affiliationUniversity of Brasilia, Health Sciences Faculty, Department of Pharmacypt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Genetics an d Morphologpt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Cellular Biologypt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciencespt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciencespt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciencespt_BR
dc.description.unidadeInstituto de Ciências Biológicas (IB)pt_BR
dc.description.unidadeDepartamento de Ciências Fisiológicas (IB CFS)pt_BR
dc.description.unidadeDepartamento de Biologia Celular (IB CEL)pt_BR
dc.description.unidadeDepartamento de Genética e Morfologia (IB GEM)pt_BR
dc.description.unidadeFaculdade de Ciências da Saúde (FS)pt_BR
dc.description.unidadeDepartamento de Farmácia (FS FAR)pt_BR
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