Título:	The peptide secreted at the water to land transition in a model amphibian has antioxidant effects
Autor(es):	Barbosa, Eder Alves Plácido, Alexandra Moreira, Daniel Carneiro Albuquerque, Lucas Fraga Friaça Dematei, Anderson Carvalho, Amandda Évelin Silva de Cabral, Wanessa F. Báo, Sônia Nair Araújo, Felipe Saldanha de Kuckelhaus, Selma Aparecida Souza Borges, Tatiana K. Portugal, Camila C. Socodato, Renato Teixeira, Cátia Lima, Filipe Camargo D. A. Batagin-Neto, Augusto Sebben, Antônio Eaton, Peter Gomes, Paula Brand, Guilherme D. Relvas, João B. Kato, Massuo J. Leite, José Roberto de Souza de Almeida
Assunto:	Anfíbio Triptofilinas Peptídeos Espectrometria de massa Stress oxidativo
Data de publicação:	10-Nov-2021
Editora:	The Royal Society
Referência:	BARBOSA, Eder Alves et al. The peptide secreted at the water to land transition in a model amphibian has antioxidant effects. Proceedings B, v. 288, n. 1962, 10 nov. 2021. DOI: https://doi.org/10.1098/rspb.2021.1531. Disponível em: https://royalsocietypublishing.org/doi/10.1098/rspb.2021.1531.
Abstract:	In addition to the morphophysiological changes experienced by amphibians during metamorphosis, they must also deal with a different set of environmental constraints when they shift from the water to the land. We found that Pithecopus azureus secretes a single peptide ([M + H]+ = 658.38 Da) at the developmental stage that precedes the onset of terrestrial behaviour. De novo peptide and cDNA sequencing revealed that the peptide, named PaT-2, is expressed in tandem and is a member of the tryptophyllins family. In silico studies allowed us to identify the position of reactive sites and infer possible antioxidant mechanisms of the compounds. Cell-based assays confirmed the predicted antioxidant activity in mammalian microglia and neuroblast cells. The potential neuroprotective effect of PaT-2 was further corroborated in FRET-based live cell imaging assays, where the peptide prevented lipopolysaccharide-induced ROS production and glutamate release in human microglia. In summary, PaT-2 is the first peptide expressed during the ontogeny of P. azureus, right before the metamorphosing froglet leaves the aquatic environment to occupy terrestrial habitats. The antioxidant activity of PaT-2, predicted by in silico analyses and confirmed by cell-based assays, might be relevant for the protection of the skin of P. azureus adults against increased O2 levels and UV exposure on land compared with aquatic environments.
Unidade Acadêmica:	Faculdade de Medicina (FMD)
DOI:	https://doi.org/10.1098/rspb.2021.1531
Versão da editora:	https://royalsocietypublishing.org/doi/10.1098/rspb.2021.1531
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