http://repositorio.unb.br/handle/10482/42820
Campo DC | Valor | Idioma |
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dc.contributor.author | Barbosa, Eder Alves | - |
dc.contributor.author | Plácido, Alexandra | - |
dc.contributor.author | Moreira, Daniel Carneiro | - |
dc.contributor.author | Albuquerque, Lucas Fraga Friaça | - |
dc.contributor.author | Dematei, Anderson | - |
dc.contributor.author | Carvalho, Amandda Évelin Silva de | - |
dc.contributor.author | Cabral, Wanessa F. | - |
dc.contributor.author | Báo, Sônia Nair | - |
dc.contributor.author | Araújo, Felipe Saldanha de | - |
dc.contributor.author | Kuckelhaus, Selma Aparecida Souza | - |
dc.contributor.author | Borges, Tatiana K. | - |
dc.contributor.author | Portugal, Camila C. | - |
dc.contributor.author | Socodato, Renato | - |
dc.contributor.author | Teixeira, Cátia | - |
dc.contributor.author | Lima, Filipe Camargo D. A. | - |
dc.contributor.author | Batagin-Neto, Augusto | - |
dc.contributor.author | Sebben, Antônio | - |
dc.contributor.author | Eaton, Peter | - |
dc.contributor.author | Gomes, Paula | - |
dc.contributor.author | Brand, Guilherme D. | - |
dc.contributor.author | Relvas, João B. | - |
dc.contributor.author | Kato, Massuo J. | - |
dc.contributor.author | Leite, José Roberto de Souza de Almeida | - |
dc.date.accessioned | 2022-02-07T13:38:40Z | - |
dc.date.available | 2022-02-07T13:38:40Z | - |
dc.date.issued | 2021-11-10 | - |
dc.identifier.citation | BARBOSA, Eder Alves et al. The peptide secreted at the water to land transition in a model amphibian has antioxidant effects. Proceedings B, v. 288, n. 1962, 10 nov. 2021. DOI: https://doi.org/10.1098/rspb.2021.1531. Disponível em: https://royalsocietypublishing.org/doi/10.1098/rspb.2021.1531. | pt_BR |
dc.identifier.uri | https://repositorio.unb.br/handle/10482/42820 | - |
dc.language.iso | Inglês | pt_BR |
dc.publisher | The Royal Society | pt_BR |
dc.rights | Acesso Aberto | pt_BR |
dc.title | The peptide secreted at the water to land transition in a model amphibian has antioxidant effects | pt_BR |
dc.type | Artigo | pt_BR |
dc.subject.keyword | Anfíbio | pt_BR |
dc.subject.keyword | Triptofilinas | pt_BR |
dc.subject.keyword | Peptídeos | pt_BR |
dc.subject.keyword | Espectrometria de massa | pt_BR |
dc.subject.keyword | Stress oxidativo | pt_BR |
dc.identifier.doi | https://doi.org/10.1098/rspb.2021.1531 | pt_BR |
dc.relation.publisherversion | https://royalsocietypublishing.org/doi/10.1098/rspb.2021.1531 | pt_BR |
dc.description.abstract1 | In addition to the morphophysiological changes experienced by amphibians during metamorphosis, they must also deal with a different set of environmental constraints when they shift from the water to the land. We found that Pithecopus azureus secretes a single peptide ([M + H]+ = 658.38 Da) at the developmental stage that precedes the onset of terrestrial behaviour. De novo peptide and cDNA sequencing revealed that the peptide, named PaT-2, is expressed in tandem and is a member of the tryptophyllins family. In silico studies allowed us to identify the position of reactive sites and infer possible antioxidant mechanisms of the compounds. Cell-based assays confirmed the predicted antioxidant activity in mammalian microglia and neuroblast cells. The potential neuroprotective effect of PaT-2 was further corroborated in FRET-based live cell imaging assays, where the peptide prevented lipopolysaccharide-induced ROS production and glutamate release in human microglia. In summary, PaT-2 is the first peptide expressed during the ontogeny of P. azureus, right before the metamorphosing froglet leaves the aquatic environment to occupy terrestrial habitats. The antioxidant activity of PaT-2, predicted by in silico analyses and confirmed by cell-based assays, might be relevant for the protection of the skin of P. azureus adults against increased O2 levels and UV exposure on land compared with aquatic environments. | pt_BR |
dc.description.unidade | Faculdade de Medicina (FMD) | - |
Aparece nas coleções: | Artigos publicados em periódicos e afins |
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