Title:	Brazilian consortium for the study on renal diseases associated with COVID-19 : a multicentric effort to understand SARS-CoV-2-related nephropathy
Authors:	Teixeira Júnior, Antonio Augusto Lima Neves, Precil Diego Miranda de Menezes Lages, Joyce Santos Cunha, Kaile de Araújo Muniz, Monique Pereira Rêgo Brito, Dyego José de Araújo Watanabe, Andréia Watanabe, Elieser Hitoshi Onuchic, Luiz Fernando Nunes, Lucas Lobato Acatauassu Coutinho Filho, Antônio Fernando Barcelos, Flávia Lara Gatto, Giuseppe Cesare Monteiro, Antonio Polido, Diego do Amaral Motta, Douglas Rafanelle Moura de Santana Leite, Thaísa de Oliveira Guedes, Felipe Leite Gomes, Orlando Vieira Valente, Lucila Maria Israel, Karla Cristina Silva Petruccelli Ladchumananandasivam, Francisco Rasiah Farias, Lígia Cristina Lopes de Marques, Igor Denizarde Bacelar Uliano, Gustavo Lemos Maramaldo, Carlos Eduardo Campos Lima Neto, Lídio Gonçalves Luchi, Weverton Machado Wanderley, David Campos Araújo, Stanley de Almeida Salgado Filho, Natalino Silva, Gyl Eanes Barros
Assunto::	Covid-19 SARS-CoV-2 Glomerulopatias Rins - doenças Microangiopatia trombótica
Issue Date:	23-Oct-2020
Publisher:	Frontiers
Citation:	TEIXEIRA JÚNIOR, Antonio Augusto Lima et al. Brazilian consortium for the study on renal diseases associated with COVID-19: a multicentric effort to understand SARS-CoV-2-related nephropathy. Frontiers in Medicine, v. 7, art. 584235, out. 2020. DOI: https://doi.org/10.3389/fmed.2020.584235. Disponível em: https://www.frontiersin.org/articles/10.3389/fmed.2020.584235/full. Acesso em: 31 dez. 2020.
Abstract:	Kidney involvement appears to be frequent in coronavirus disease 2019 (COVID-19). Despite this, information concerning renal involvement in COVID-19 is still scarce. Several mechanisms appear to be involved in the complex relationship between the virus and the kidney. Also, different morphological patterns have been described in the kidneys of patients with COVID-19. For some authors, however, this association may be just a coincidence. To investigate this issue, we propose assessing renal morphology associated with COVID-19 at the renal pathology reference center of federal university hospitals in Brazil. Data will come from a consortium involving 17 federal university hospitals belonging to Empresa Brasileira de Serviços Hospitalares (EBSERH) network, as well as some state hospitals and an autopsy center. All biopsies will be sent to the referral center for renal pathology of the EBSERH network. The data will include patients who had coronavirus disease, both alive and deceased, with or without pre-existing kidney disease. Kidney biopsies will be analyzed by light, fluorescence, and electron microscopy. Furthermore, immunohistochemical (IHC) staining for various inflammatory cells (i.e., cells expressing CD3, CD20, CD4, CD8, CD138, CD68, and CD57) as well as angiotensin-converting enzyme 2 (ACE2) will be performed on paraffinized tissue sections. In addition to ultrastructural assays, in situ hybridization (ISH), IHC and reverse transcription-polymerase chain reaction (RT-PCR) will be used to detect Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) in renal tissue. For the patients diagnosed with Collapsing Glomerulopathy, peripheral blood will be collected for apolipoprotein L-1 (APOL1) genotyping. For patients with thrombotic microangiopathy, thrombospondin type 1 motif, member 13 (ADAMTS13), antiphospholipid, and complement panel will be performed. The setting of this study is Brazil, which is second behind the United States in highest confirmed cases and deaths. With this complete approach, we hope to help define the spectrum and impact, whether immediate or long-term, of kidney injury caused by SARS-CoV-2.
Licença::	Copyright © 2020 Teixeira Júnior, Neves, Lages, Cunha, Muniz, Brito, Watanabe, Watanabe, Onuchic, Nunes, Coutinho Filho, Barcelos, Gatto, Monteiro, Polido, Motta, Leite, Guedes, Gomes, Valente, Israel, Ladchumananandasivam, de Farias, Marques, Uliano, Maramaldo, Neto, Luchi, Wanderley, Araújo, Salgado Filho and Silva. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
DOI:	https://doi.org/10.3389/fmed.2020.584235
Appears in Collections:	Artigos publicados em periódicos e afins UnB - Covid-19

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