Título:	Reference genes for RT-qPCR analysis in Musa acuminata genotypes contrasting in resistance to Fusarium oxysporum f. sp. cubense subtropical race 4
Autor(es):	Costa, Érica de Castro Bastos, Lucas Santos Gomes, Taísa Godoy Miller, Robert Neil Gerard
Afiliação do autor:	Universidade de Brasília, Instituto de Ciências Biológicas, Departamento de Fitopatologia Universidade de Brasília, Instituto de Ciências Biológicas, Departamento de Fitopatologia Universidade de Brasília, Instituto de Ciências Biológicas, Departamento de Biologia Celular Universidade de Brasília, Instituto de Ciências Biológicas, Departamento de Biologia Celular
Assunto:	Banana Banana - genética Fungos fitopatogênicos
Data de publicação:	14-Jul-2024
Editora:	Springer Nature
Referência:	COSTA, Érica de Castro et al. Reference genes for RT-qPCR analysis in Musa acuminata genotypes contrasting in resistance to Fusarium oxysporum f. sp. cubense subtropical race 4. Scientific Reports, v. 14, 16578, 2024. DOI: https://doi.org/10.1038/s41598-024-67538-0. Disponível em: https://www.nature.com/articles/s41598-024-67538-0#data-availability. Acesso em:
Abstract:	Banana (Musa spp.) is the most widely consumed fruit globally. Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense (Foc), is a highly threatening disease to banana production. Resistance genes to Foc exist in wild Musa genotypes such as Musa acuminata subsp. burmannicoides var. Calcutta 4. Whilst real-time PCR (RT-qPCR) is appropriate for accurate analysis of gene expression in pathways involved in host defence responses, reference genes with stable expression under specifc biotic stress conditions and host tissue types are necessary for normalization of sample variation. In this context, the stability in potential host reference genes ACT1, APT, EF1α, GAPDH, αTUB, RAN, UBIQ1, UBIQ2, βTUB1, βTUB3, L2 and ACTA1 was evaluated in total RNA samples from root tissues in Calcutta 4 (resistant) and Musa sp. cultivar Prata-anã (susceptible) extracted during interaction with Foc subtropical race 4 (STR4). Expression stability was calculated using the algorithms geNorm, NormFinder and BestKeeper. βTUB3 and L2 were identifed as the most stable in Calcutta 4, with ACTA1 and GAPDH the most stable in Prata-anã. These reference genes for analysis of gene expression modulation in the Musa-Foc STR4 pathosystem are fundamental for advancing understanding of host defence responses to this important pathogen.
Unidade Acadêmica:	Instituto de Ciências Biológicas (IB) Departamento de Fitopatologia (IB FIT) Departamento de Biologia Celular (IB CEL)
Programa de pós-graduação:	Programa de Pós-Graduação em Fitopatologia
Licença:	(CC BY) Open Access Tis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
DOI:	https://doi.org/10.1038/s41598-024-67538-0
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