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dc.contributor.authorCosta, Érica de Castro-
dc.contributor.authorBastos, Lucas Santos-
dc.contributor.authorGomes, Taísa Godoy-
dc.contributor.authorMiller, Robert Neil Gerard-
dc.date.accessioned2024-09-30T19:36:08Z-
dc.date.available2024-09-30T19:36:08Z-
dc.date.issued2024-07-14-
dc.identifier.citationCOSTA, Érica de Castro et al. Reference genes for RT-qPCR analysis in Musa acuminata genotypes contrasting in resistance to Fusarium oxysporum f. sp. cubense subtropical race 4. Scientific Reports, v. 14, 16578, 2024. DOI: https://doi.org/10.1038/s41598-024-67538-0. Disponível em: https://www.nature.com/articles/s41598-024-67538-0#data-availability. Acesso em:pt_BR
dc.identifier.urihttp://repositorio.unb.br/handle/10482/50480-
dc.language.isoengpt_BR
dc.publisherSpringer Naturept_BR
dc.rightsAcesso Abertopt_BR
dc.titleReference genes for RT-qPCR analysis in Musa acuminata genotypes contrasting in resistance to Fusarium oxysporum f. sp. cubense subtropical race 4pt_BR
dc.typeArtigopt_BR
dc.subject.keywordBananapt_BR
dc.subject.keywordBanana - genéticapt_BR
dc.subject.keywordFungos fitopatogênicospt_BR
dc.rights.license(CC BY) Open Access Tis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.pt_BR
dc.identifier.doihttps://doi.org/10.1038/s41598-024-67538-0pt_BR
dc.description.abstract1Banana (Musa spp.) is the most widely consumed fruit globally. Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense (Foc), is a highly threatening disease to banana production. Resistance genes to Foc exist in wild Musa genotypes such as Musa acuminata subsp. burmannicoides var. Calcutta 4. Whilst real-time PCR (RT-qPCR) is appropriate for accurate analysis of gene expression in pathways involved in host defence responses, reference genes with stable expression under specifc biotic stress conditions and host tissue types are necessary for normalization of sample variation. In this context, the stability in potential host reference genes ACT1, APT, EF1α, GAPDH, αTUB, RAN, UBIQ1, UBIQ2, βTUB1, βTUB3, L2 and ACTA1 was evaluated in total RNA samples from root tissues in Calcutta 4 (resistant) and Musa sp. cultivar Prata-anã (susceptible) extracted during interaction with Foc subtropical race 4 (STR4). Expression stability was calculated using the algorithms geNorm, NormFinder and BestKeeper. βTUB3 and L2 were identifed as the most stable in Calcutta 4, with ACTA1 and GAPDH the most stable in Prata-anã. These reference genes for analysis of gene expression modulation in the Musa-Foc STR4 pathosystem are fundamental for advancing understanding of host defence responses to this important pathogen.pt_BR
dc.contributor.affiliationUniversidade de Brasília, Instituto de Ciências Biológicas, Departamento de Fitopatologiapt_BR
dc.contributor.affiliationUniversidade de Brasília, Instituto de Ciências Biológicas, Departamento de Fitopatologiapt_BR
dc.contributor.affiliationUniversidade de Brasília, Instituto de Ciências Biológicas, Departamento de Biologia Celularpt_BR
dc.contributor.affiliationUniversidade de Brasília, Instituto de Ciências Biológicas, Departamento de Biologia Celularpt_BR
dc.description.unidadeInstituto de Ciências Biológicas (IB)pt_BR
dc.description.unidadeDepartamento de Fitopatologia (IB FIT)pt_BR
dc.description.unidadeDepartamento de Biologia Celular (IB CEL)pt_BR
dc.description.ppgPrograma de Pós-Graduação em Fitopatologiapt_BR
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