Título:	Insights into the microRNA landscape of Rhodnius prolixus, a vector of Chagas disease
Autor(es):	Santiago, Paula Beatriz Bentes, Kaio Luís da Silva Silva, Waldeyr Mendes Cordeiro da Praça, Yanna Reis Charneau, Sébastien Chaouch, Soraya Grellier, Philippe Ferraz, Marcos Antônio dos Santos Silva Bastos, Izabela Marques Dourado Santana, Jaime Martins de Araújo, Carla Nunes de
Afiliação do autor:	University of Brasília, Institute of Biology, Department of Cell Biology, Pathogen-Host Interface Laboratory University of Brasília, Institute of Biology, Department of Cell Biology, Pathogen-Host Interface Laboratory Federal Institute of Goiás, Formosa, Brazil University of Brasília, Institute of Biology, Department of Cell Biology, Pathogen-Host Interface Laboratory University of Brasília, Institute of Biology, Department of Cell Biology, Laboratory of Protein Chemistry and Biochemistry Muséum National d’Histoire Naturelle, UMR 7245 Molécules de Communication et Adaptation des Micro-organismes Muséum National d’Histoire Naturelle, UMR 7245 Molécules de Communication et Adaptation des Micro-organismes University of Brasília, Institute of Biology, Department of Cell Biology, Scientific Illustration Center University of Brasília, Institute of Biology, Department of Cell Biology, Pathogen-Host Interface Laboratory University of Brasília, Institute of Biology, Department of Cell Biology, Pathogen-Host Interface Laboratory University of Brasília, Institute of Biology, Department of Cell Biology, Pathogen-Host Interface Laboratory Faculty of Ceilândia, University of Brasília
Assunto:	Vetores Chagas, Doença de MicroRNA Triatomíneos
Data de publicação:	12-Ago-2023
Editora:	Nature Research
Referência:	SANTIAGO, Paula Beatriz et al. Insights into the microRNA landscape of Rhodnius prolixus, a vector of Chagas disease. Scientific Reports, [S. l.], v. 13, art. n. 13120, 2023. DOI: https://doi.org/10.1038/s41598-023-40353-9. Disponível em: https://www.nature.com/articles/s41598-023-40353-9. Acesso em: 02 jul. 2024.
Abstract:	The growing interest in microRNAs (miRNAs) over recent years has led to their characterization in numerous organisms. However, there is currently a lack of data available on miRNAs from triatomine bugs (Reduviidae: Triatominae), which are the vectors of the protozoan parasite Trypanosoma cruzi, the causative agent of Chagas disease. A comprehensive understanding of the molecular biology of vectors provides new insights into insect-host interactions and insect control approaches, which are key methods to prevent disease incidence in endemic areas. In this work, we describe the miRNome profiles from gut, hemolymph, and salivary gland tissues of the Rhodnius prolixus triatomine. Small RNA sequencing data revealed abundant expression of miRNAs, along with tRNA- and rRNA-derived fragments. Fifty-two mature miRNAs, previously reported in Ecdysozoa, were identified, including 39 ubiquitously expressed in the three tissues. Additionally, 112, 73, and 78 novel miRNAs were predicted in the gut, hemolymph, and salivary glands, respectively. In silico prediction showed that the top eight most highly expressed miRNAs from salivary glands potentially target human blood-expressed genes, suggesting that R. prolixus may modulate the host’s gene expression at the bite site. This study provides the first characterization of miRNAs in a Triatominae species, shedding light on the role of these crucial regulatory molecules.
Unidade Acadêmica:	Instituto de Ciências Biológicas (IB) Departamento de Biologia Celular (IB CEL) Núcleo de Educação Científica (IB NECBio) Faculdade UnB Ceilândia (FCE)
Programa de pós-graduação:	Programa de Pós-Graduação em Ciências Médicas
Licença:	Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
DOI:	https://doi.org/10.1038/s41598-023-40353-9
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