Title:	Challenges in the detection of polymyxin resistance : from today to the future
Authors:	Rubens, Rebeca Siqueira Arruda, Isabel de Souza Andrade Almeida, Rosane Mansan Nóbrega, Yanna Karla de Medeiros Carneiro, Maiara dos Santos Dalmolin, Tanise Vendruscolo
metadata.dc.identifier.orcid:	https://orcid.org/0000-0001-8545-069X https://orcid.org/0000-0001-9975-6470
metadata.dc.contributor.affiliation:	Universidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia, Laboratório de Microbiologia e Imunologia Clínica (LabMIC) Universidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia, Laboratório de Microbiologia e Imunologia Clínica (LabMIC) Universidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia, Laboratório de Microbiologia e Imunologia Clínica (LabMIC) Universidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia, Laboratório de Microbiologia e Imunologia Clínica (LabMIC) Centro de Medicina Laboratorial, Farroupilha Universidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia, Laboratório de Microbiologia e Imunologia Clínica (LabMIC)
Assunto::	Suscetibilidade a antimicrobianos Polimixinas Bactérias gram-negativas
Issue Date:	4-Jan-2024
Publisher:	MDPI
Citation:	RUBENS, Rebeca Siqueira et al. Challenges in the detection of polymyxin resistance: from today to the future. Microorganisms, v. 12, n. 1, 2024. DOI: https://doi.org/10.3390/microorganisms12010101. Disponível em: https://www.mdpi.com/2076-2607/12/1/101.
Abstract:	Antimicrobial resistance is known to be one of the greatest global threats to human health, and is one of the main causes of death worldwide. In this scenario, polymyxins are last-resort antibiotics to treat infections caused by multidrug-resistant bacteria. Currently, the reference test to evaluate the susceptibility of isolates to polymyxins is the broth microdilution method; however, this technique has numerous complications and challenges for use in laboratory routines. Several phenotypic methods have been reported as being promising for implementation in routine diagnostics, including the BMD commercial test, rapid polymyxin NP test, polymyxin elution test, culture medium with polymyxins, and the Polymyxin Drop Test, which require materials for use in routines and must be easy to perform. Furthermore, Sensititre®, molecular tests, MALDI-TOF MS, and Raman spectroscopy present reliable results, but the equipment is not found in most microbiology laboratories. In this context, this review discusses the main laboratory methodologies that allow the detection of resistance to polymyxins, elucidating the challenges and perspectives.
metadata.dc.description.unidade:	Faculdade de Ciências da Saúde (FS) Departamento de Farmácia (FS FAR)
Licença::	© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
DOI:	https://doi.org/10.3390/microorganisms12010101
Appears in Collections:	Artigos publicados em periódicos e afins

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