Título:	Evaluation of biocompatibility, anti-inflammatory, and antinociceptive activities of pequi oil-based nanoemulsion in in vitro and in vivo models
Autor(es):	Pinheiro, Andréia Camargo Ombredane, Alicia Simalie Pinheiro, Willie Oliveira Andrade, Laise R. Silva, Vitória R. P. Felice, Gisela J. Alves, Débora S. Albernaz, Aryanne F. Silveira, Ariene Pandolfo Lima, Milena Campelo Freitas Veiga-Junior, Valdir Florencio Gomes, Thamis F. S. Damasceno, Emanuel Adelino Medeiros Souza, Fabiane Hiratsuka Veiga Souza, Paulo Eduardo Narcizo de Báo, Sônia Nair Duarte, Eliza Carla Barroso Carneiro, Marcella Lemos Brettas Azevedo, Ricardo Bentes Funez, Mani Indiana Joanitti, Graziella Anselmo
ORCID:	https://orcid.org/0000-0003-1365-7602 https://orcid.org/0000-0002-9873-3098 https://orcid.org/0000-0002-2137-9588
Afiliação do autor:	University of Brasilia, Ceilândia Sul, Laboratory of Bioactive Compounds and Nanobiotechnology University of Brasilia, Institute of Biological Sciences, Post-Graduation Program in Nanoscience and Nanobiotechnology University of Brasilia, Ceilândia Sul, Laboratory of Bioactive Compounds and Nanobiotechnology University of Brasilia, Institute of Biological Sciences, Post-Graduation Program in Nanoscience and Nanobiotechnology University of Brasilia, Ceilândia Sul, Laboratory of Bioactive Compounds and Nanobiotechnology University of Brasilia, School of Ceilândia, Postgraduate Program in Health Sciences and Technologies University of Brasilia, Ceilândia Sul, Laboratory of Bioactive Compounds and Nanobiotechnology University of Brasilia, Institute of Biological Sciences, Post-Graduation Program in Nanoscience and Nanobiotechnology University of Brasilia, Ceilândia Sul, Laboratory of Bioactive Compounds and Nanobiotechnology University of Brasilia, Ceilândia Sul, Laboratory of Bioactive Compounds and Nanobiotechnology University of Brasilia, Ceilândia Sul, Laboratory of Bioactive Compounds and Nanobiotechnology University of Brasilia, Institute of Biological Sciences, Department of Cell Biology, Laboratory of Protein Chemistry and Biochemistry University of Brasilia, School of Ceilândia, Postgraduate Program in Health Sciences and Technologies University of Brasilia, Institute of Biological Sciences, Department of Cell Biology, Laboratory of Microscopy and Microanalysis Military Institute of Engineering, Chemistry Section, Rio de Janeiro Military Institute of Engineering, Chemistry Section, Rio de Janeiro University of Brasilia, Ceilândia Sul, Laboratory of Bioactive Compounds and Nanobiotechnology University of Brasilia, Institute of Biological Sciences, Post-Graduation Program in Nanoscience and Nanobiotechnology Regional Hospital of Taguatinga, Health Department, Nucleus of Cytopathology and Anatomic Pathology University of Brasilia, Institute of Biological Sciences, Department of Cell Biology, Laboratory of Protein Chemistry and Biochemistry Universidade de Brasília, Faculdade de Ceilândia, Faculdade de Ciências Farmacêuticas University of Brasília, Institute of Physics, Laboratory of Electron Paramagnetic Resonance University of Brasilia, Institute of Biological Sciences, Department of Cell Biology, Laboratory of Microscopy and Microanalysis University of Brasília, Faculty of Medicine, Department of Pathology University of Brasilia, Ceilândia Sul, Laboratory of Bioactive Compounds and Nanobiotechnology University of Brasília, Faculty of Gama, Post-Graduation Program in Biomedical Engineering University of Brasilia, Institute of Biological Sciences, Post-Graduation Program in Nanoscience and Nanobiotechnology University of Brasilia, School of Ceilândia, Postgraduate Program in Health Sciences and Technologies University of Brasilia, School of Ceilândia, Nursing Course University of Brasilia, Ceilândia Sul, Laboratory of Bioactive Compounds and Nanobiotechnology University of Brasilia, Institute of Biological Sciences, Post-Graduation Program in Nanoscience and Nanobiotechnology University of Brasilia, School of Ceilândia, Postgraduate Program in Health Sciences and Technologies
Assunto:	Nanoemulsão Óleo de pequi Anti-inflamatórios Nanobiotecnologia
Data de publicação:	30-Nov-2022
Editora:	MDPI
Referência:	PINHEIRO, Andréia C. et al. Evaluation of biocompatibility, anti-inflammatory, and antinociceptive activities of pequi oil-based nanoemulsion in in vitro and in vivo models. Nanomaterials, [S. l.], v. 12, n. 23, 4260, 2022. DOI: https://doi.org/10.3390/nano12234260. Disponível em: https://www.mdpi.com/2079-4991/12/23/4260. Acesso em: 08 fev. 2024.
Abstract:	Pequi oil (Caryocar brasiliense) contains bioactive compounds capable of modulating the inflammatory process; however, its hydrophobic characteristic limits its therapeutic use. The encapsulation of pequi oil in nanoemulsions can improve its biodistribution and promote its immunomodulatory effects. Thus, the objective of the present study was to formulate pequi oil-based nanoemulsions (PeNE) to evaluate their biocompatibility, anti-inflammatory, and antinociceptive effects in in vitro (macrophages—J774.16) and in vivo (Rattus novergicus) models. PeNE were biocompatible, showed no cytotoxic and genotoxic effects and no changes in body weight, biochemistry, or histology of treated animals at all concentrations tested (90–360 µg/mL for 24 h, in vitro; 100–400 mg/kg p.o. 15 days, in vivo). It was possible to observe antinociceptive effects in a dose-dependent manner in the animals treated with PeNE, with a reduction of 27 and 40% in the doses of 100 and 400 mg/kg of PeNE, respectively (p < 0.05); however, the treatment with PeNE did not induce edema reduction in animals with carrageenan-induced edema. Thus, the promising results of this study point to the use of free and nanostructured pequi oil as a possible future approach to a preventive/therapeutic complementary treatment alongside existing conventional therapies for analgesia.
Unidade Acadêmica:	Faculdade UnB Planaltina (FUP) Instituto de Ciências Biológicas (IB) Departamento de Biologia Celular (IB CEL) Faculdade UnB Ceilândia (FCE) Curso de Farmácia (FCE-FAR) Faculdade de Medicina (FM) Faculdade de Ciências e Tecnologias em Engenharia (FCTE) – Campus UnB Gama
Programa de pós-graduação:	Programa de Pós-Graduação em Nanociência e Nanobiotecnologia Programa de Pós-Graduação em Ciências e Tecnologias em Saúde
Licença:	Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
DOI:	https://doi.org/10.3390/nano12234260
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