Título:	Use of an on/of tetracycline riboswitch to control protein production in Komagataella phafi
Autor(es):	Vieira, Igor Patrick Vasconcelos Pimentel, Felipe Seixas Arreguy Coelho, Cintia Marques De Marco, Janice Lisboa Moraes, Lidia Maria Pepe de Torres, Fernando Araripe Gonçalves
ORCID:	https://orcid.org/0000-0002-3650-7271
Afiliação do autor:	Universidade de Brasília, Instituto de Ciências Biológicas, Laboratório de Biologia Molecular Universidade Federal do Rio de Janeiro, Instituto de Bioquímica Médica, Laboratório de Biotecnologia de Leveduras Universidade de Brasília, Instituto de Ciências Biológicas, Laboratório de Biologia Sintética Universidade de Brasília, Instituto de Ciências Biológicas, Laboratório de Biologia Molecular Universidade de Brasília, Instituto de Ciências Biológicas, Laboratório de Biologia Molecular Universidade de Brasília, Instituto de Ciências Biológicas, Laboratório de Biologia Molecular
Assunto:	Leveduras Tetraciclina Proteínas
Data de publicação:	21-Nov-2023
Editora:	AMB Express
Referência:	VIEIRA, Igor Patrick Vasconcelos et al. Use of an on/of tetracycline riboswitch to control protein production in Komagataella phafi. AMB Express, v. 13, 131, 2023. DOI: https://doi.org/10.1186/s13568-023-01637-5. Disponível em: https://amb-express.springeropen.com/articles/10.1186/s13568-023-01637-5. Acesso em: 07 fev. 2024.
Abstract:	The methylotrophic yeast Komagataella phafi is one of the most important microbial platforms to produce recom binant proteins. Despite its importance in the context of industrial biotechnology, the use of synthetic biology approaches in K. phafi is hampered by the fact that few genetic tools are available for precise control of gene expres sion in this system. In this work, we used an RNA aptamer activated by tetracycline to modulate protein production at the translational level. Using lacZ as gene reporter, we have demonstrated signifcant reduction of the heterolo gous protein upon addition of tetracycline. Furthermore, this genetic control device was applied for the control of Ku70p. This protein is involved in non-homologous recombination and the control of its production paves the way for the development of strains exhibiting higher rates of homologous recombination.
Unidade Acadêmica:	Instituto de Ciências Biológicas (IB) Departamento de Biologia Celular (IB CEL) Departamento de Genética e Morfologia (IB GEM)
Licença:	© The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
DOI:	https://doi.org/10.1186/s13568-023-01637-5
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