Título:	Fish oil nanoemulsion supplementation attenuates bleomycin-induced pulmonary fibrosis BALB/c Mice
Autor(es):	Souza, Danielle Galdino de Santos, Débora Silva Simon, Karina Smidt Morais, José Athayde Vasconcelos Coelho, Luísa Coutinho Pacheco, Thyago José Arruda Azevedo, Ricardo Bentes Bocca, Anamélia Lorenzetti Melo-Silva, César Augusto Longo, João Paulo Figueiró
ORCID:	https://orcid.org/0000-0003-3432-0769 https://orcid.org/0000-0003-2395-7748 https://orcid.org/0000-0002-8090-0644 https://orcid.org/0000-0002-2137-9588 https://orcid.org/0000-0003-3323-5300 https://orcid.org/0000-0002-3544-6999
Afiliação do autor:	University of Brasília, Institute of Biological Science, Nanobiotechnology Laboratory, Genetics & Morphology Department University of Brasília, Institute of Biological Science, Nanobiotechnology Laboratory, Genetics & Morphology Department University of Brasília, Institute of Biological Science, Applied Immunology Laboratory, Cell Biology Department University of Brasília, Institute of Biological Science, Nanobiotechnology Laboratory, Genetics & Morphology Department University of Brasília, Institute of Biological Science, Applied Immunology Laboratory, Cell Biology Department University of Brasília, Institute of Biological Science, Nanobiotechnology Laboratory, Genetics & Morphology Department University of Brasília, Institute of Biological Science, Nanobiotechnology Laboratory, Genetics & Morphology Department University of Brasília, Institute of Biological Science, Applied Immunology Laboratory, Cell Biology Department University of Brasília, Faculty of Medicine, Respiratory Physiology Laboratory University of Brasília, Institute of Biological Science, Nanobiotechnology Laboratory, Genetics & Morphology Department
Assunto:	Nanoemulsão Fibrose pulmonar Medicamentos Óleos de peixe
Data de publicação:	2022
Editora:	MDPI
Referência:	SOUZA, Danielle Galdino de Souza; SANTOS, Débora Silva Santos; SIMON, Karina Smidt Simon; MORAIS, José Athayde Vasconcelos COELHO, Luísa Coutinho Coelho; PACHECO, Thyago José Arruda; AZEVEDO, Ricardo Bentes; BOCCA, Anamélia Lorenzetti; MELO-SILVA, César Augusto Melo-Silva; LONGO, João Paulo Figueiró. Fish oil nanoemulsion supplementation attenuates bleomycin-induced pulmonary fibrosis BALB/c Mice. Nanomaterials, [S.l.], v. 12, 1683, 2022. DOI: https://doi.org/10.3390/nano12101683. Disponível em: https://www.mdpi.com/2079-4991/12/10/1683. Acesso em: 09 jul. 2023.
Abstract:	Diets rich in omega-3 or -6 fatty acids will produce different profiles for cell membranes phospholipid constitutions. Omegas 3 and 6 are part of the diet and can modulate the inflammatory profile. We evaluated the effects of the oral absorption of fish oil, when associated with a lipid nanoemulsion in an experimental pulmonary inflammatory model. Pulmonary fibrosis is a disease associated with excessive extracellular matrix deposition. We determined to investigate the morphophysiological mechanisms in mice that were pretreated after induction with bleomycin (BLM). The pretreatment was for 21 days with saline solution, sunflower oil (SO), fish oil (FO), and fish oil nanoemulsion (NEW3). The animals received a daily dose of 50 mg/Kg of docosahexaenoic acid DHA and 10 mg/Kg eicosapentaenoic (EPA) (100 mg/Kg), represented by a daily dose of 40 µL of NEW3. The blank group was treated with the same amount daily (40 µL) during the 21 days of pretreatment. The animals were treated with SO and FO, 100 mg/Kg (containing 58 mg/Kg of polyunsaturated fats/higher% linoleic acid) and 100 mg/Kg (50 mg/Kg of DHA and 10 mg/Kg EPA), respectively. A single dose of 5 mg/mL (50 µL) bleomycin sulfate, by the intratracheal surgical method in BALB/cAnNTac (BALB/c). NEW3 significantly reduced fibrotic progression, which can be evidenced by the protection from loss of body mass, increase in respiratory incursions per minute, decreased spacing of alveolar septa, decreased severity of fibrosis, and changes in the respiratory system. NEW3 attenuated the inflammatory changes developed in the experimental model of pulmonary fibrosis, while group SO showed a significant increase in inflammatory changes. This concluded that the presented results demonstrated that is possible to positively modulate the immune and inflamamtory response to an external agressor, by changing the nutitional intake of specific fatty acids, such as omega-3 placed in fish oil. Moreover, these benefits can be improved by the nanoencapsulation of fish oil in lipid nanoemulsions.
Unidade Acadêmica:	Instituto de Ciências Biológicas (IB) Departamento de Genética e Morfologia (IB GEM) Departamento de Biologia Celular (IB CEL) Faculdade de Medicina (FMD)
Programa de pós-graduação:	Programa de Pós-Graduação em Nanociência e Nanobiotecnologia
Licença:	© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
DOI:	https://doi.org/10.3390/nano12101683
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