Titre:	The classical photoactivated drug 8-methoxypsoralen and related compounds are effective without UV light irradiation against glioma cells
Auteur(s):	Oliveira, Diêgo Madureira de Lima, Rute Maria Ferreira Velozo, Jorge Clarencio de Eudes da Silva Amorim, Ilza Alves de Mota, Tales Henrique Andrade da Costa, Silvia Lima Pittella-Silva, Fabio El-Bachá, Ramon dos Santos
metadata.dc.contributor.affiliation:	Universidade de Brasília, Faculdade UnB Ceilândia Universidade de Brasília, Faculdade de Ciências da Saúde, Laboratório de Patologia Molecular do Câncer Universidade Federal da Bahia,Instituto de Ciências da Saúde, Laboratório de Neuroquímica e Biologia Celular Fiocruz, Centro de Pesquisa Gonçalo Moniz, Bahia Escola de Medicina e Saúde Pública da Bahia Universidade Federal da Bahia, Faculdade de Farmácia, LAPEMM
Assunto::	Glioma Medicamentos
Date de publication:	2016
Editeur:	Elsevier
Référence bibliographique:	OLIVEIRA, Diêgo Madureira de et al. The classical photoactivated drug 8-methoxypsoralen and related compounds are effective without UV light irradiation against glioma cells. Neurochemistry International, v. 99, p. 33-41, out. 2016. DOI: https://doi.org/10.1016/j.neuint.2016.06.004.
Abstract:	Currently, there is no effective therapy for high grade gliomas. 8-Methoxypsoralen (8-MOP) is a compound used in the treatment of skin diseases combined with UV light irradiation. In this work, rat glioma C6 cells, normal astrocytes and human glioblastoma GL-15 cells comprised an in vitro model to evaluate the antitumor activity of 8-MOP. We found that 8-MOP promoted a time- and concentration-dependent reduction of cell viability in tumor, but not in normal cells. This effect was more evident in log-phase growing culture, indicating antiproliferative activity, which was confirmed by colony formation assay. Long-term effect of 8-MOP at low concentration was also attested. The concentrations used in the tests (0.02–0.4 mM) were lower than plasmatic concentration found in patients. Despite the treatment leads to considerable morphological changes and apoptosis when used at high concentrations, 8-MOP did not promote cell cycle arrest, change in migration pattern neither necrosis. In addition, we evaluated the effect of 8-MOP in MDA-MB-231, CT-26 and SCC-3 cell lines, derived from other kind of primary tumors, and found that CT-26 cells did not respond to 8-MOP treatment, indicating that this compound does not act through a generic mechanism. Coumarin derivatives structurally related to 8-MOP were screened for its antitumor potential and presented different patterns of biological activity, and then it was possible to suggest the relevance of 8-MOP molecular structure for antiproliferative action. Therefore, 8-MOP, a drug with an outstanding record of safety, and related coumarins are good prototypes for development of a new class of anti-glioma drugs.
DOI:	https://doi.org/10.1016/j.neuint.2016.06.004
metadata.dc.relation.publisherversion:	https://www.sciencedirect.com/science/article/pii/S0197018616301498?via%3Dihub
Collection(s) :	Artigos publicados em periódicos e afins

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