Skip navigation
Veuillez utiliser cette adresse pour citer ce document : http://repositorio.unb.br/handle/10482/43597
Fichier(s) constituant ce document :
Il n'y a pas de fichiers associés à ce document.
Titre: RT-rtPCR quantification of circulating microRNAs in plasma and serum samples from healthy domestic cats
Auteur(s): Scalon, Marcela C.
Martins, Christine S.
Ferreira, Gabriel G.
Schlemmer, Franciele
Almeida, Ricardo Titze de
Paludo, Giane Regina
metadata.dc.identifier.orcid: https://orcid.org/0000-0002-3686-8413
https://orcid.org/ 0000-0002-9112-1718
Assunto:: Sangue
Diagnóstico
Gato - doenças
MicroRNA
Date de publication: 23-jui-2021
Editeur: SAGE
Référence bibliographique: SCALON, Marcela C. et al. RT-rtPCR quantification of circulating microRNAs in plasma and serum samples from healthy domestic cats. Journal of Veterinary Diagnostic Investigation, p. 1-5, 2021. DOI: https://doi.org/10.1177/10406387211034843.
Abstract: MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at a post-transcriptional level by silencing targeted messenger RNA (mRNA). Most studies concerning miRNA expression use solid tissue samples. However, circulating miRNAs from different body fluids have recently emerged as diagnostic and prognostic molecules, given that they hold informative value and have increased stability in cell-free form. Blood sampling of cats can be challenging given their small body size and because they often experience distress when handled. We quantified miR-20a, -192, -365, -15b-5p, and -16-5p from plasma and serum samples of 10 healthy domestic cats. Our RT-rtPCR procedure used 100 µL of either plasma or serum samples as sources of biomarker molecules. However, serum provided higher amounts of miRNA than plasma samples, with a p < 0.0001 for miR-20a and p < 0.0002 for miR-16-5p.
metadata.dc.description.unidade: Faculdade de Agronomia e Medicina Veterinária (FAV)
DOI: https://doi.org/10.1177/10406387211034843
metadata.dc.relation.publisherversion: https://journals.sagepub.com/doi/10.1177/10406387211034843
Collection(s) :Artigos publicados em periódicos e afins

Affichage détaillé " class="statisticsLink btn btn-primary" href="/jspui/handle/10482/43597/statistics">



Tous les documents dans DSpace sont protégés par copyright, avec tous droits réservés.