Título:	Molecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2) : new tentative species within the order picornavirales
Autor(es):	Nery, Flávia M. B. Melo, Fernando Lucas Boiteux, Leonardo Silva Ribeiro, Simone G. Resende, Renato de Oliveira Orílio, Anelise Franco Batista, Josiane Goulart Lima, Mirtes Freitas Carvalho, Rita de Cássia Pereira
ORCID:	https://orcid.org/0000-0002-5791-8002 https://orcid.org/0000-0002-4965-1424
Assunto:	Vírus de plantas Metagenômica Picornavirales
Data de publicação:	27-Ago-2020
Editora:	MDPI
Referência:	NERY, Flávia M. B. et al. Molecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2): new tentative species within the order picornavirales. Viruses, v. 12, n. 9, 950, 2020. DOI: https://doi.org/10.3390/v12090950. Disponível em: https://www.mdpi.com/1999-4915/12/9/950. Acesso em: 22 out. 2020.
Abstract:	In a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel virus species remains to be discovered.
Licença:	© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
DOI:	https://doi.org/10.3390/v12090950
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