| DC Field | Value | Language |
|---|
| dc.contributor.author | Nery, Flávia M. B. | - |
| dc.contributor.author | Melo, Fernando Lucas | - |
| dc.contributor.author | Boiteux, Leonardo Silva | - |
| dc.contributor.author | Ribeiro, Simone G. | - |
| dc.contributor.author | Resende, Renato de Oliveira | - |
| dc.contributor.author | Orílio, Anelise Franco | - |
| dc.contributor.author | Batista, Josiane Goulart | - |
| dc.contributor.author | Lima, Mirtes Freitas | - |
| dc.contributor.author | Carvalho, Rita de Cássia Pereira | - |
| dc.date.accessioned | 2020-10-22T13:35:09Z | - |
| dc.date.available | 2020-10-22T13:35:09Z | - |
| dc.date.issued | 2020-08-27 | - |
| dc.identifier.citation | NERY, Flávia M. B. et al. Molecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2): new tentative species within the order picornavirales. Viruses, v. 12, n. 9, 950, 2020. DOI: https://doi.org/10.3390/v12090950. Disponível em: https://www.mdpi.com/1999-4915/12/9/950. Acesso em: 22 out. 2020. | pt_BR |
| dc.identifier.uri | https://repositorio.unb.br/handle/10482/39576 | - |
| dc.language.iso | Inglês | pt_BR |
| dc.publisher | MDPI | pt_BR |
| dc.rights | Acesso Aberto | pt_BR |
| dc.title | Molecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2) : new tentative species within the order picornavirales | pt_BR |
| dc.type | Artigo | pt_BR |
| dc.subject.keyword | Vírus de plantas | pt_BR |
| dc.subject.keyword | Metagenômica | pt_BR |
| dc.subject.keyword | Picornavirales | pt_BR |
| dc.rights.license | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | pt_BR |
| dc.identifier.doi | https://doi.org/10.3390/v12090950 | pt_BR |
| dc.description.abstract1 | In a systematic field survey for plant-infecting viruses, leaf tissues were collected from
trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and
sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534
and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their
genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants
hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated
virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species.
The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited
a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was
tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the
amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2
clustered in distinct groups, and both viruses were tentatively assigned as new members of the order
Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50
ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1
and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s)
of these novel virus species remains to be discovered. | pt_BR |
| dc.identifier.orcid | https://orcid.org/0000-0002-5791-8002 | pt_BR |
| dc.identifier.orcid | https://orcid.org/0000-0002-4965-1424 | pt_BR |
| Appears in Collections: | Artigos publicados em periódicos e afins
|
