http://repositorio.unb.br/handle/10482/25599
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Titre: | Implementação de um sistema de eletroforese capilar com detecção de fluorescência induzida por laser Development of a laser induced fluorescence detection scheme for capillary electrophoresis |
Auteur(s): | Santos, Marcia R. Tavares, Marina F. M. Rubim, Joel C. |
Assunto:: | Eletroforese capilar Detecção de fluorescência induzida por laser Ftalocianina Zn sulfonada |
Date de publication: | 2000 |
Editeur: | Sociedade Brasileira de Química |
Référence bibliographique: | Quím. Nova,v.23,n.5,p.585-589,2000 |
Résumé: | A capillary electrophoresis system using laser induced fluorescence detection is described. A Raman system equipped with a microscope has been used to focus the laser beam on the capillary giving a lateral resolution of 1.5 mm. The fluorescence signal of the analyte (ZnPcTS - tetrasulfonated zinc-phthalocyanine) was collected by the microscope objectives and analysed by a monochromator with confocal characteristics equipped with a CCD detector. Electropherograms obtained with this system were compared to those obtained on a commercial instrument, showing that the described system presents a lower detection limit and better resolution. |
metadata.dc.description.unidade: | Em processamento |
DOI: | https://dx.doi.org/10.1590/S0100-40422000000500003 |
Collection(s) : | Artigos publicados em periódicos e afins |
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