http://repositorio.unb.br/handle/10482/25027
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ARTIGO_NonhemocyteSourcesSelected.pdf | 343,56 kB | Adobe PDF | Voir/Ouvrir |
Titre: | Nonhemocyte sources of selected lysosomal enzymes in Biomphalaria glabrata, B. tenagophila and B. straminea (Mollusca: Pulmonata) |
Auteur(s): | Rodrick, Gary E. Monteiro, W. Sodeman Júnior, W. A. |
Assunto:: | Biomphalaria glabrata Enzimas |
Date de publication: | sep-1981 |
Editeur: | Instituto Oswaldo Cruz, Ministério da Saúde |
Référence bibliographique: | RODRICK, Gary E.; MONTEIRO, W.; SODEMAN JUNIOR, W. A. Nonhemocyte sources of selected lysosomal enzymes in Biomphalaria glabrata, B. tenagophila and B. straminea (Mollusca: Pulmonata). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 76, n. 3, p. 293-297, jul./set. 1981. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761981000300007&lng=en&nrm=iso>. Acesso em: 16 nov. 2017. doi: http://dx.doi.org/10.1590/S0074-02761981000300007. |
Résumé: | Foram determinadas, na massa cefalopedal e na glândula digestiva de Biomphalaria glabrata de Touros (Rio Grande do Norte) B. tenagophila de Cacapava (Sao Paulo) e B. straminea de Monsenhor Gil (Piauí), as atividades específicas das seguintes enzimas: fosfatase acida, fosfatase alcalina, beta-glucuronidase, lisozima, transaminase glutâmico-oxalacetica e transaminase glutâmico-piruvica. As seis enzimas referidas foram detectadas no sobrenadante a 3000g. Ambas as enzimas citoplasmaticas - transaminases glutamico-oxalacetica e glutamico-piruvica - mostraram as atividades específicas mais altas. No caso das quatro enzimas hidrolíticas, a beta-glucuronidase revelou a mais alta atividade específica, enquanto a lisozima revelou a mais baixa. E admitido que todas as seis enzimas sao produzidas por celulas presentes na massa cefalopedal e na glândula digestiva das tres especies de moluscos examinadas. |
Abstract: | The specific activities of acid phosphatase, alkaline phosphatase, β-glucuronidase, lysozymes, glutamate-oxalacetate transaminase and glutamate-pyruvate transaminate were determined in the head-foot and digestive gland of Brazilian Biomphalaria glabrata (Touros), B. tenagophila (Caçapava) and B. straminea (Monsenhor Gil). All six enzymes were detected inthe 3000g supernatant. Both cytoplasmic enzymes, glutamate-oxalacetate and glutamate-pyruvate transaminase exhibited the highest specific activities. In the case of the four hydrolytic enzymes assayed, β-glucuronidase exhibited the highest specific activity while lysozyme showed the lowest activity. All six enzymes are thought to be produced by cells within the head-foot and digestive gland of B. glabrata, B. tenagophila and B. straminea. |
Licença:: | Memórias do Instituto Oswaldo Cruz - All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License (CC BY NC 4.0). Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761981000300007&lng=en&nrm=iso. Acesso em: 16 nov. 2017. |
DOI: | http://dx.doi.org/10.1590/S0074-02761981000300007 |
Collection(s) : | Artigos publicados em periódicos e afins |
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