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dc.contributor.authorHermenegildo, Pollyane da Silva-
dc.contributor.authorFreitas, Rodrigo Galvão de-
dc.contributor.authorCascardo, Renan de Souza-
dc.contributor.authorGuimarães, Lúcio Mauro Silva-
dc.contributor.authorPacheco, Jorge Luis Badel-
dc.contributor.authorAlfenas‑Zerbini, Poliane-
dc.contributor.authorMarques, Abi Soares dos Anjos-
dc.contributor.authorAlfenas, Acelino Couto-
dc.contributor.authorFerreira, Marisa Alvares da Silva Velloso-
dc.date.accessioned2025-09-17T10:57:28Z-
dc.date.available2025-09-17T10:57:28Z-
dc.date.issued2022-11-22-
dc.identifier.citationHERMENEGILDO, Pollyane da Silva et al. Sensitive detection, quantifcation, and monitoring of Erwinia psidii colonization of guava plants using intercalating dye‑based real‑time PCR. Tropical Plant Pathology, [S. l.], v. 48, p. 375–383, 2023. DOI: https://doi.org/10.1007/s40858-022-00542-9pt_BR
dc.identifier.urihttp://repositorio.unb.br/handle/10482/52452-
dc.language.isoengpt_BR
dc.publisherSpringer Naturept_BR
dc.rightsAcesso Restritopt_BR
dc.titleSensitive detection, quantifcation, and monitoring of Erwinia psidii colonization of guava plants using intercalating dye‑based real‑time PCRpt_BR
dc.typeArtigopt_BR
dc.subject.keywordGoiaba - doenças e pragaspt_BR
dc.subject.keywordGoiaba - bactériaspt_BR
dc.subject.keywordDiagnóstico bacteriológicopt_BR
dc.identifier.doihttps://doi.org/10.1007/s40858-022-00542-9pt_BR
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s40858-022-00542-9pt_BR
dc.description.abstract1Bacterial blight caused by Erwinia psidii is considered an important disease of the guava crop in Brazil. The disease has been disseminated to diferent geographic areas due to the movement of infected but asymptomatic propagating plant material. Consequently, methods showing high specifcity and sensitivity for early detection of latent infections are required to aid in the establishment of the use of pathogen-free seedlings and propagating material. In this study, an intercalating dye-based real-time PCR (qPCR) method using newly designed species-specifc primers targeting the recA gene sequence was developed. Primer specifcity was frst confrmed in silico and then by PCR amplifcation using DNA from strains of a collection of E. psidii and other plant-associated bacterial species. DNA from strains of other bacterial species obtained from uninfected guava and eucalypt leaves or from other plant species were not amplifed. When bacterial suspensions and purifed DNA were used in qPCR, detection limits were 103 CFU mL−1 and 102 genomic units µL−1, respectively. Using qPCR, E. psidii was detected in 100% of samples from symptomatic and in 57.1% of samples from asymptomatic trees collected in four guava orchards. The qPCR method allowed quantifcation of E. psidii populations in infected tissue of varieties Pedro Sato and Sassaoka as well as confrmation of the previously reported E. psidii acropetal and basipetal movement inside the plant. This new detection method with improved sensitivity has great potential not only for implementing disease diagnosis in guava and eucalypt nurseries and orchards but also for investigating relevant aspects of E. psidii life cycle and epidemiology.pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4283-122Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-1298-9149pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-1397-8086pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-5021-6684pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-8203-5244pt_BR
dc.identifier.orcidhttps://orcid.org/0009-0008-4751-1170pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-7776-3362pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0677-861Xpt_BR
dc.contributor.affiliationUniversidade de Brasília, Departamento de Fitopatologiapt_BR
dc.contributor.affiliationUniversidade Federal de Viçosa, Departamento de Fitopatologiapt_BR
dc.contributor.affiliationUniversidade Federal de Viçosa, Departamento de Fitopatologiapt_BR
dc.contributor.affiliationUniversidade Federal de Viçosa, Departamento de Fitopatologiapt_BR
dc.contributor.affiliationUniversidade Federal de Viçosa, Departamento de Fitopatologiapt_BR
dc.contributor.affiliationUniversidade Federal de Viçosa, Departamento de Microbiologiapt_BR
dc.contributor.affiliationEmbrapa Recursos Genéticos e Biotecnologia, Gestão da Estação Quarentenária de Germoplasma Vegetalpt_BR
dc.contributor.affiliationUniversidade Federal de Viçosa, Departamento de Fitopatologiapt_BR
dc.contributor.affiliationUniversidade de Brasília, Departamento de Fitopatologiapt_BR
dc.description.unidadeInstituto de Ciências Biológicas (IB)pt_BR
dc.description.unidadeDepartamento de Fitopatologia (IB FIT)pt_BR
dc.description.ppgPrograma de Pós-Graduação em Fitopatologiapt_BR
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