Title:	Diversidade de vírus de ssDNA e alfasatélites em solanáceas e caracterização molecular de três novas espécies de Begomovirus em tomateiro
Authors:	Guimarães, Giovana Curcio
Orientador(es)::	Carvalho, Rita de Cássia Pereira
Assunto::	Tomate - doenças e pragas Vírus de plantas
Issue Date:	24-Jul-2024
Data de defesa::	19-Dec-2023
Citation:	GUIMARÃES, Giovana Curcio. Diversidade de vírus de ssDNA e alfasatélites em solanáceas e caracterização molecular de três novas espécies de Begomovirus em tomateiro. 2023. 106 f., il. Dissertação (Mestrado em Fitopatologia) — Universidade de Brasília, Brasília, 2023.
Abstract:	The Solanaceae family is a diverse botanical family that encompasses several species of angiosperm plants. The tomato (Solanum lycopersicum L.) is one of the most important vegetables both economically and socially in Brazil. The productivity levels of this crop suffer recurrent fluctuations due to several factors, among which diseases such as begomoviruses (caused by a complex of begomovirus species) stand out. These pathogens have one or two single-stranded circular DNA molecules (presenting the components DNA–A and DNA–B) and predominate in tomato plants in the New World, being transmitted by different cryptic species of the Bemisia tabaci complex. The advent of High Throughput Sequencing (HTS) technology has led to the discovery of new viral species associated with tomato cultivation. In this context, the objective of this work is to study viral diversity in tomato and weeds from the Solanaceae family. For this, in chapter two, one hundred and twenty-nine (129) leaf samples from tomato and weed plants exhibiting typical begomovirus symptoms (apical chlorosis, golden mosaic and chlorotic spots) were collected in the North (15), Northeast (43), South (11) Midwest (45), Southeast (18) regions. Collections were carried out from 2003 to 2022, with leaf samples being analyzed from various representatives of the Solanaceae family. The extracted total DNA was used as template for rolling circle amplification (RCA). To confirm the presence of begomoviruses through PCR assays using degenerate primers for DNA–A (PAR1c496 and PAL1v1978) and for DNA–B (PBL and CRC). After this step, RCA of the 129 samples were used to form a pool and sequenced on an Ilumina NovaSeq 6000 platform. 41,659 contigs were obtained, 172 of which showed, after analysis with RefSeqViral, identities with begomovirus (168), topilevirus (1) and gemycircularvirus (1) and two alphasatellites. Sixteen of the 168 begomovirus contigs showed nucleotide identity below 91%, being consistent with the current taxonomic criteria for defining new species within the genus Begomovirus. In chater 3 another three contigs (C16, C21 and C230) showed a genomic organization typical of bipartite begomoviruses and were considered potential new species within the Begomovirus genus. Using HTS, it was possible to observe the presence of high levels of genetic variability and diversity of begomoviruses in members of the Solanaceae family, indicating an important role as viral reservoirs. Research actions are being conducted aimed at producing infectious clones to uncover the range of hosts as well as sources of resistance against these new viruses.
metadata.dc.description.unidade:	Instituto de Ciências Biológicas (IB) Departamento de Fitopatologia (IB FIT)
Description:	Dissertação (mestrado) — Universidade de Brasília, Instituto de Ciências Biológicas, Departamento de Fitopatologia, 2023.
metadata.dc.description.ppg:	Programa de Pós-Graduação em Fitopatologia
Licença::	A concessão da licença deste item refere-se ao termo de autorização impresso assinado pelo autor com as seguintes condições: Na qualidade de titular dos direitos de autor da publicação, autorizo a Universidade de Brasília e o IBICT a disponibilizar por meio dos sites www.bce.unb.br, www.ibict.br, http://hercules.vtls.com/cgi-bin/ndltd/chameleon?lng=pt&skin=ndltd sem ressarcimento dos direitos autorais, de acordo com a Lei nº 9610/98, o texto integral da obra disponibilizada, conforme permissões assinaladas, para fins de leitura, impressão e/ou download, a título de divulgação da produção científica brasileira, a partir desta data.
Appears in Collections:	Teses, dissertações e produtos pós-doutorado

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