RT-rtPCR quantification of circulating microRNAs in plasma and serum samples from healthy domestic cats

Scalon, Marcela C.; Martins, Christine S.; Ferreira, Gabriel G.; Schlemmer, Franciele; Almeida, Ricardo Titze de; Paludo, Giane Regina

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dc.contributor.author	Scalon, Marcela C.	-
dc.contributor.author	Martins, Christine S.	-
dc.contributor.author	Ferreira, Gabriel G.	-
dc.contributor.author	Schlemmer, Franciele	-
dc.contributor.author	Almeida, Ricardo Titze de	-
dc.contributor.author	Paludo, Giane Regina	-
dc.date.accessioned	2022-05-02T14:48:37Z	-
dc.date.available	2022-05-02T14:48:37Z	-
dc.date.issued	2021-07-23	-
dc.identifier.citation	SCALON, Marcela C. et al. RT-rtPCR quantification of circulating microRNAs in plasma and serum samples from healthy domestic cats. Journal of Veterinary Diagnostic Investigation, p. 1-5, 2021. DOI: https://doi.org/10.1177/10406387211034843.	pt_BR
dc.identifier.uri	https://repositorio.unb.br/handle/10482/43597	-
dc.language.iso	Inglês	pt_BR
dc.publisher	SAGE	pt_BR
dc.rights	Acesso Restrito	pt_BR
dc.title	RT-rtPCR quantification of circulating microRNAs in plasma and serum samples from healthy domestic cats	pt_BR
dc.type	Artigo	pt_BR
dc.subject.keyword	Sangue	pt_BR
dc.subject.keyword	Diagnóstico	pt_BR
dc.subject.keyword	Gato - doenças	pt_BR
dc.subject.keyword	MicroRNA	pt_BR
dc.identifier.doi	https://doi.org/10.1177/10406387211034843	pt_BR
dc.relation.publisherversion	https://journals.sagepub.com/doi/10.1177/10406387211034843	pt_BR
dc.description.abstract1	MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at a post-transcriptional level by silencing targeted messenger RNA (mRNA). Most studies concerning miRNA expression use solid tissue samples. However, circulating miRNAs from different body fluids have recently emerged as diagnostic and prognostic molecules, given that they hold informative value and have increased stability in cell-free form. Blood sampling of cats can be challenging given their small body size and because they often experience distress when handled. We quantified miR-20a, -192, -365, -15b-5p, and -16-5p from plasma and serum samples of 10 healthy domestic cats. Our RT-rtPCR procedure used 100 µL of either plasma or serum samples as sources of biomarker molecules. However, serum provided higher amounts of miRNA than plasma samples, with a p < 0.0001 for miR-20a and p < 0.0002 for miR-16-5p.	pt_BR
dc.identifier.orcid	https://orcid.org/0000-0002-3686-8413	pt_BR
dc.identifier.orcid	https://orcid.org/ 0000-0002-9112-1718	pt_BR
dc.description.unidade	Faculdade de Agronomia e Medicina Veterinária (FAV)	-
Collection(s) :	Artigos publicados em periódicos e afins