Title:	Análise do impacto da deleção do gene p94 (ac134) de autographa californica multiple nucleopolyhedovirus
Authors:	Andrade, Miguel de Souza
Orientador(es)::	Ribeiro, Bergmann Morais
Coorientador(es)::	Melo, Fernando Lucas
Assunto::	Baculovírus Vírus derivado da oclusão Nucleocapsídeos
Issue Date:	30-Jul-2016
Data de defesa::	2-Mar-2016
Citation:	ANDRADE, Miguel de Souza. Análise do impacto da deleção do gene p94 (ac134) de autographa californica multiple nucleopolyhedovirus. 2015. 83 f., il. Dissertação (Mestrado em Biologia Molecular)—Universidade de Brasília, Brasília, 2015.
Abstract:	The baculoviruses are a group of insect viruses with large dsDNA genomes, and their primary infection is triggered by rod-shaped enveloped virions (ODV) embedded in a crystalline protein matrix. Each virion may contain single (SNPV) or multiple nucleocapsids (MNPV) within an envelope, depending on the viral species. The genetic basis of this phenotype has not been identified and it does not seem to be correlated with baculovirus phylogeny. However, some closely related baculoviruses, such as BmNPV, BomaNPV and AcMNPV, have discordant phenotypes and may help to identify candidate genes. Therefore, we compared the genome of these viruses and found a unique gene (p94) that was present in BomaNPV and AcMNPV and was absent in BmNPV. This gene was the most significant difference among the BmNPV and the BomaNPN and AcMNPV genomes. To analyze whether this gene was related to the MNPV phenotype we constructed three recombinant baculoviruses: vAc-pol-p94-del (p94 disrupted), vAc-pol-p94-ha-rep (p94 repaired with -HA tag) and vAc-pol (control). We found that the P94 was detected at 6 h post-infection (p.i.) in the cytoplasm of infected cells with maximum accumulation at 24 h p.i. The lack of complete p94 gene (including the exclusion of the origin of DNA replication located there) in the AcMNPV genome resulted in a delay in DNA replication, BV production and IE1 expression. OB production in vitro was also decreased. Moreover, the ODV nucleocapsid envelopment was not chanced. Therefore, the p94 gene of AcMNPV is not an essential gene for viral DNA replication and establishment of viral infection. Its acquisition in some baculoviruses genomes might give a selective advantage for the virus.
metadata.dc.description.unidade:	Instituto de Ciências Biológicas (IB) Departamento de Biologia Celular (IB CEL)
Description:	Dissertação (mestrado)—Universidade de Brasília, Instituto de Ciências Biológicas, Programa de Pós Graduação em Biologia Molecular, 2015.
metadata.dc.description.ppg:	Programa de Pós-Graduação em Biologia Molecular
Licença::	A concessão da licença deste item refere-se ao termo de autorização impresso assinado pelo autor com as seguintes condições: Na qualidade de titular dos direitos de autor da publicação, autorizo a Universidade de Brasília e o IBICT a disponibilizar por meio dos sites www.bce.unb.br, www.ibict.br, http://hercules.vtls.com/cgi-bin/ndltd/chameleon?lng=pt&skin=ndltd sem ressarcimento dos direitos autorais, de acordo com a Lei nº 9610/98, o texto integral da obra disponibilizada, conforme permissões assinaladas, para fins de leitura, impressão e/ou download, a título de divulgação da produção científica brasileira, a partir desta data.
DOI:	http://dx.doi.org/10.26512/2016.03.D.21114
Appears in Collections:	Teses, dissertações e produtos pós-doutorado

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