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dc.contributor.authorCardoso, Samuel Leite-
dc.contributor.authorSouza, Paula Monteiro de-
dc.contributor.authorRodrigues, Kelly Barreto-
dc.contributor.authorMota, Isabella de Souza-
dc.contributor.authorFerreira Filho, Edivaldo Ximenes-
dc.contributor.authorFávaro, Léia Cecilia de Lima-
dc.contributor.authorAraujo, Felipe Saldanha de-
dc.contributor.authorHomem de Mello, Mauricio-
dc.contributor.authorPessoa, Adalberto-
dc.contributor.authorDuarte, Dâmaris Silveira-
dc.contributor.authorBazzo, Yris Maria Fonseca-
dc.contributor.authorBatista, Pérola de Oliveira Magalhães Dias-
dc.date.accessioned2024-09-20T15:17:41Z-
dc.date.available2024-09-20T15:17:41Z-
dc.date.issued2023-09-20-
dc.identifier.citationCARDOSO, Samuel Leite et al. L-Asparaginase type II from fusarium proliferatum: heterologous expression and in silico analysis. Pharmaceutics, [S. l.], v. 15, n. 9, 2352, 2023. DOI: https://doi.org/10.3390/pharmaceutics15092352. Disponível em: https://www.mdpi.com/1999-4923/15/9/2352. Acesso em: 20 set. 2024.pt_BR
dc.identifier.urihttp://repositorio.unb.br/handle/10482/50395-
dc.language.isoengpt_BR
dc.publisherMDPIpt_BR
dc.rightsAcesso Abertopt_BR
dc.titleL-Asparaginase type II from fusarium proliferatum : heterologous expression and in silico analysispt_BR
dc.typeAnaispt_BR
dc.subject.keywordL -asparaginasept_BR
dc.subject.keywordSilos graneleiros - análisept_BR
dc.subject.keywordFungos fitopatogênicospt_BR
dc.rights.licenseCopyright: © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).pt_BR
dc.identifier.doihttps://doi.org/10.3390/pharmaceutics15092352pt_BR
dc.description.abstract1The search for new drug-producing microorganisms is one of the most promising situations in current world scientific scenarios. The use of molecular biology as well as the cloning of protein and compound genes is already well established as the gold standard method of increasing productivity. Aiming at this increase in productivity, this work aims at the cloning, purification and in silico analysis of l-asparaginase from Fusarium proliferatum in Komagataella phaffii (Pichia pastoris) protein expression systems. The l-asparaginase gene (NCBI OQ439985) has been cloned into Pichia pastoris strains. Enzyme production was analyzed via the quantification of aspartic B-hydroxamate, followed by purification on a DEAE FF ion exchange column. The in silico analysis was proposed based on the combined use of various technological tools. The enzymatic activity found intracellularly was 2.84 IU/g. A purification factor of 1.18 was observed. The in silico analysis revealed the position of five important amino acid residues for enzymatic activity, and likewise, it was possible to predict a monomeric structure with a C-score of 1.59. The production of the enzyme l-asparaginase from F. proliferatum in P. pastoris was demonstrated in this work, being of great importance for the analysis of new methodologies in search of the production of important drugs in therapy.pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-1824-2211pt_BR
dc.identifier.orcidhttps://orcid.org/0009-0002-9014-3488pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-4947-8753pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-3260-0310pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4541-9177pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-3260-0310pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4541-9177pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-5268-8690pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-1851-5224pt_BR
dc.contributor.affiliationUniversity of Brasilia, Health Science Schoolpt_BR
dc.contributor.affiliationUniversity of Brasilia, Health Science Schoolpt_BR
dc.contributor.affiliationBrazilian Agricultural Research Corporation—EMBRAPA Agroenergiapt_BR
dc.contributor.affiliationUniversity of Brasilia, Health Science Schoolpt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciencespt_BR
dc.contributor.affiliationBrazilian Agricultural Research Corporation—EMBRAPA Agroenergiapt_BR
dc.contributor.affiliationUniversity of Brasilia, Health Science Schoolpt_BR
dc.contributor.affiliationUniversity of São Paulo, Department of Biochemical and Pharmaceutical Technologypt_BR
dc.contributor.affiliationUniversity of Brasilia, Health Science Schoolpt_BR
dc.contributor.affiliationUniversity of Brasilia, Health Science Schoolpt_BR
dc.contributor.affiliationUniversity of Brasilia, Health Science Schoolpt_BR
dc.contributor.affiliationUniversity of Brasilia, Health Science Schoolpt_BR
dc.description.unidadeFaculdade de Ciências da Saúde (FS)pt_BR
dc.description.unidadeInstituto de Ciências Biológicas (IB)pt_BR
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