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dc.contributor.authorFreitas, Marcela Medeiros de-
dc.contributor.authorSouza, Paula Monteiro de-
dc.contributor.authorHomem de Mello, Mauricio-
dc.contributor.authorFonseca-Bazzo, Yris Maria-
dc.contributor.authorSilveira, Dâmaris-
dc.contributor.authorFerreira Filho, Edivaldo Ximenes-
dc.contributor.authorPessoa Junior, Adalberto-
dc.contributor.authorSarker, Dipak-
dc.contributor.authorTimson, David-
dc.contributor.authorInácio, João-
dc.contributor.authorMagalhães, Pérola de Oliveira-
dc.date.accessioned2024-07-16T12:41:47Z-
dc.date.available2024-07-16T12:41:47Z-
dc.date.issued2022-06-14-
dc.identifier.citationFREITAS, Marcela et al. L-Asparaginase from Penicillium sizovae produced by a recombinant Komagataella phaffii strain. Pharmaceuticals, [S. l.], v. 15, n.6, 2022. DOI: https://doi.org/10.3390/ph15060746.pt_BR
dc.identifier.urihttp://repositorio2.unb.br/jspui/handle/10482/48896-
dc.language.isoengpt_BR
dc.publisherMDPIpt_BR
dc.rightsAcesso Abertopt_BR
dc.titleL-Asparaginase from Penicillium sizovae produced by a recombinant Komagataella phaffii strainpt_BR
dc.typeArtigopt_BR
dc.subject.keywordL-Asparaginasept_BR
dc.subject.keywordLeucemia linfoblástica aguda (LLA)pt_BR
dc.subject.keywordClonagempt_BR
dc.subject.keywordFungospt_BR
dc.identifier.doihttps://doi.org/10.3390/ph15060746pt_BR
dc.description.abstract1: L-asparaginase is an important enzyme in the pharmaceutical field used as treatment for acute lymphoblastic leukemia due to its ability to hydrolyze L-asparagine, an essential amino acid synthesized by normal cells, but not by neoplastic cells. Adverse effects of L-asparaginase formulations are associated with its glutaminase activity and bacterial origin; therefore, it is important to find new sources of L-asparaginase produced by eukaryotic microorganisms with low glutaminase activity. This work aimed to identify the L-asparaginase gene sequence from Penicillium sizovae, a filamentous fungus isolated from the Brazilian Savanna (Cerrado) soil with low glutaminase activity, and to biosynthesize higher yields of this enzyme in the yeast Komagataella phaffii. The L-asparaginase gene sequence of P. sizovae was identified by homology to L-asparaginases from species of Penicillium of the section Citrina: P. citrinum and P. steckii. Partial L-asparaginase from P. sizovae, lacking the periplasmic signaling sequence, was cloned, and expressed intracellularly with highest enzymatic activity achieved by a MUT+ clone cultured in BMM expression medium; a value 5-fold greater than that obtained by native L-asparaginase in P. sizovae cells. To the best of our knowledge, this is the first literature report of the heterologous production of an L-asparaginase from a filamentous fungus by a yeast.pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-6112-9903pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-1824-2211pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4541-9177pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-1230-3207pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-1851-5224pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-4947-8753pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-5268-8690pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-2912-415Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0985-8818pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8011-6940pt_BR
dc.contributor.affiliationUniversity of Brasilia, Health Sciences Schoolpt_BR
dc.contributor.affiliationUniversity of Brasilia, Health Sciences Schoolpt_BR
dc.contributor.affiliationUniversity of Brasilia, Health Sciences Schoolpt_BR
dc.contributor.affiliationUniversity of Brasilia, Health Sciences Schoolpt_BR
dc.contributor.affiliationUniversity of Brasilia, Health Sciences Schoolpt_BR
dc.contributor.affiliationUniversity of Brasilia, Institute of Biological Sciencespt_BR
dc.contributor.affiliationUniversity of Sao Paulo, Department of Biochemical and Pharmaceutical Technologypt_BR
dc.contributor.affiliationUniversity of Brighton, School of Pharmacy and Biomolecular Sciencespt_BR
dc.contributor.affiliationUniversity of Brighton, School of Pharmacy and Biomolecular Sciencespt_BR
dc.contributor.affiliationUniversity of Brighton, School of Pharmacy and Biomolecular Sciencespt_BR
dc.contributor.affiliationUniversity of Brasilia, Health Sciences Schoolpt_BR
dc.description.unidadeFaculdade de Ciências da Saúde (FS)pt_BR
dc.description.unidadeDepartamento de Farmácia (FS FAR)pt_BR
dc.description.unidadeInstituto de Ciências Biológicas (IB)pt_BR
dc.description.unidadeDepartamento de Biologia Celular (IB CEL)pt_BR
dc.description.ppgPrograma de Pós-Graduação em Ciências Farmacêuticaspt_BR
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