Título:	Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss
Autor(es):	Malard, Patrícia Furtado Peixer, Mauricio Antônio da Silva Grázia, João Gabriel Viana de Brunel, Hilana dos Santos Sena Féres, Luiz Fernando Rodrigues Villarroel, Carla Lujan Pereira Siqueira, Luiz Gustavo Bruno Dode, Margot Alves Nunes Pogue, Robert Viana, João Henrique Moreira Carvalho, Juliana Lott de
ORCID:	https://orcid.org/0000-0002-3073-4748 https://orcid.org/0000-0002-9118-7768 https://orcid.org/0000-0002-9976-681X https://orcid.org/0000-0002-9425-1593 https://orcid.org/0000-0001-7566-2859 https://orcid.org/0000-0002-5125-9798 https://orcid.org/0000-0002-2800-5829 https://orcid.org/0000-0002-1096-0457 https://orcid.org/0000-0002-8789-3512 https://orcid.org/0000-0002-3742-2368 https://orcid.org/0000-0002-1423-0523
Afiliação do autor:	Universidade Católica de Brasília Bio Biotecnologia da Reprodução Animal Universidade Católica de Brasília Bio Biotecnologia da Reprodução Animal Cenatte Embrioes Bio Biotecnologia da Reprodução Animal Universidade de Alfenas Bio Biotecnologia da Reprodução Animal Empresa Brasileira de Pesquisa Agropecuária - EMBRAPA Gado de Leite Empresa Brasileira de Pesquisa Agropecuária - EMBRAPA Recursos Genéticos e Biotecnologia Universidade Católica de Brasília Empresa Brasileira de Pesquisa Agropecuária - EMBRAPA Recursos Genéticos e Biotecnologia Universidade Católica de Brasília Universidade de Brasília
Assunto:	Reprodução animal Células-tronco mesenquimais Bovino - embrião
Data de publicação:	15-Mai-2020
Editora:	Springer Nature
Referência:	MALARD, Patrícia F. et al. Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss. Scientific Reports, v. 10, art. n. 8018, 2020. DOI: https://doi.org/10.1038/s41598-020-64810-x. Disponível em: https://www.nature.com/articles/s41598-020-64810-x. Acesso em: 19 mar. 2024.
Abstract:	Valuable female cattle are continuously subject to follicular puncture (ovum pick-up - OPU). This technique is commonly used for in-vitro embryo production, but may result in ovarian lesion. Mesenchymal stem cells (MSC) ameliorate the function of injured tissues, but their use to treat ovarian lesions in cattle has not been established. We investigated whether a local injection of MSC would reduce the negative efects of repeated OPU under acute and chronic scenarios in bovines. First, we performed four OPU sessions and injected 2.5×106 MSCs immediately after the 4th OPU procedure (n=5). The treated organs (right ovary) were compared to their saline-treated counterparts (left), and presented superior production of oocytes and embryos in the three following OPU sessions (P<0.05). Then, cows with progressive fertility loss went through three OPU sessions. Animals received MSC, saline, or MSC+FSH in both ovaries after the frst OPU. In the two following OPU sessions, the MSC and MSC +FSH - treated groups failed to present any signifcant alteration in the number of oocytes and embryos compared to saline-treated animals. Thus, MSC have benefcial efects on the fertility of OPU lesioned cows, but not in cows with cystic ovarian disease and chronic ovarian lesions.
Unidade Acadêmica:	Faculdade de Medicina (FM)
Licença:	(CC BY) Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Cre ative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not per mitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
DOI:	https://doi.org/10.1038/s41598-020-64810-x
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