Título:	Euphorbia tirucalli latex loaded polymer nanoparticles : synthesis, characterization, in vitro release and in vivo antinociceptive action
Autor(es):	Rodrigues, Marina Lima Gomes, Anderson de Jesus Funez, Mani Indiana Marques, Mariane Aparecida da Silva Lunardi, Claure Nain
ORCID:	https://orcid.org/0000-0001-8712-4417
Afiliação do autor:	University of Brasilia, Campus Ceilandia, Laboratory of Photochemistry and Nanobiotechnology University of Brasilia, Program in Nanoscience and Nanobiotechnology University of Brasilia, Campus Ceilandia, Laboratory of Photochemistry and Nanobiotechnology University of Brasilia, Program in Nanoscience and Nanobiotechnology University of Brasilia, Campus Ceilandia, Sciences and Technologies in Health Program University of Brasilia, Campus Ceilandia, Sciences and Technologies in Health Program University of Brasilia, Campus Ceilandia, Sciences and Technologies in Health Program University of Brasilia, Campus Ceilandia, Laboratory of Photochemistry and Nanobiotechnology University of Brasilia, Program in Nanoscience and Nanobiotechnology University of Brasilia, Campus Ceilandia, Laboratory of Photochemistry and Nanobiotechnology
Assunto:	Nanopartículas Medicamentos Látex
Data de publicação:	29-Nov-2022
Editora:	Plos One
Referência:	RODRIGUES, Marina Lima et al. Euphorbia tirucalli latex loaded polymer nanoparticles: synthesis, characterization, in vitro release and in vivo antinociceptive action. Plos One, [S. l.], v. 17, n. 11, e0274432, 2022. DOI: https://doi.org/10.1371/journal.pone.0274432.Disponível em: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0274432. Acesso em: 01 fev. 2024.
Abstract:	The encapsulation of drugs in micro and nanocarriers has helped to resolve mechanisms of cellular resistance and decrease drug side effects as well. In this study, poly(D,L-lactide-co glycolide) (PLGA) was used to encapsulate the Euphol active substance-containing latex from Euphorbia tirucalli (E-latex). The nanoparticles (NP) were prepared using the solvent evaporation method and the physical and chemical properties were evaluated using spec trophotometric techniques. FTIR was used to prove the formation of the ester bond between the E-latex and PLGA-NP. The UV-Vis spectroscopic technique was used to show that more than 75% of the latex was encapsulated; the same technique was used to determine the release profile of the compound at different pH values, as well as determining the speed with which the process occurs through kinetic models, and it was observed that the best adjustments occurred for the Korsmeyer-Peppas model and the Higuchi model. The DLS technique was used to determine the diameter of the particles produced as well as their zeta potential (ZP). The sizes of the particles varied from 497 to 764 nm, and it was observed that the increase in E-latex concentration causes a reduction in the diameter of the NP and an increase in the ZP (-1.44 to -22.7 mV), due to more functional groups from latex film being adsorbed to the NPs surfaces. The thermogravimetric experiments exhibit the glass transition temperatures (Tg) that is appropriate for the use of formulated NPs as a stable drug delivery device before use. The in vivo activity of E-NPs (30 and 100 mg/Kg/p.o.) was tested against carrageenan-induced mechanical hypernociception. The data demonstrated a significantly antinociceptive effect for E-NPs, suggesting that E-latex nanoencapsulation preserved its desired properties.
Unidade Acadêmica:	Faculdade UnB Ceilândia (FCE) Colegiado de Bases Biológicas e da Saúde (FCE-BASES) Instituto de Ciências Biológicas (IB)
Programa de pós-graduação:	Programa de Pós-Graduação em Nanociência e Nanobiotecnologia Programa de Pós-Graduação em Ciências e Tecnologias em Saúde
Licença:	Copyright: © 2022 Rodrigues et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI:	https://doi.org/10.1371/journal.pone.0274432
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