| Campo DC | Valor | Lengua/Idioma |
|---|
| dc.contributor.author | Favero, Fernanda | - |
| dc.contributor.author | Tolentino, Terezinha Alves | - |
| dc.contributor.author | Fernandes, Vinicius | - |
| dc.contributor.author | Treptow, Werner | - |
| dc.contributor.author | Pereira, Alex Leite | - |
| dc.contributor.author | Machado, Angelo Henrique Lira | - |
| dc.date.accessioned | 2023-11-28T13:14:42Z | - |
| dc.date.available | 2023-11-28T13:14:42Z | - |
| dc.date.issued | 2023-06-15 | - |
| dc.identifier.citation | FAVERO, Fernanda et al. α-Alkylidene δ-lactones inhibit quorum sensing phenotypes in Chromobacterium strain CV026 showing interaction with the CviR receptor. RSC Advances, [S. l.], v. 13, p. 18045-18057, 2023. DOI: https://doi.org/10.1039/D3RA01975F. Disponível em: https://pubs.rsc.org/en/content/articlelanding/2023/ra/d3ra01975f. Acesso em: 27 nov. 2023. | pt_BR |
| dc.identifier.uri | http://repositorio2.unb.br/jspui/handle/10482/46916 | - |
| dc.language.iso | eng | pt_BR |
| dc.publisher | The Royal Society of Chemistry | pt_BR |
| dc.rights | Acesso Aberto | pt_BR |
| dc.title | α-Alkylidene δ-lactones inhibit quorum sensing phenotypes in Chromobacterium strain CV026 showing interaction with the CviR receptor | pt_BR |
| dc.type | Artigo | pt_BR |
| dc.subject.keyword | Bactérias | pt_BR |
| dc.subject.keyword | Lactonas | pt_BR |
| dc.rights.license | (CC BY NC) This article is licensed under aCreative Commons Attribution-NonCommercial 3.0 Unported Licence. | pt_BR |
| dc.identifier.doi | https://doi.org/10.1039/D3RA01975F | pt_BR |
| dc.description.abstract1 | Disruption of bacterial quorum sensing (QS) is presented as a promising strategy to overcome clinically relevant and phytopathogenic bacteria. This work presents α-alkylidene δ-lactones as new chemical scaffolds that inhibit the biosynthesis of violacein in the biosensor strain Chromobacterium CV026. Three molecules displayed higher than 50% violacein reduction when tested at concentrations lower than 625 µM. The most active α-alkylidene δ-lactone inhibited the hydrolysis of chitin concomitantly with the inhibition of violacein production in CV026, suggesting the disruption of its QS machinery. Further, RT-qPCR and competition experiments showed this molecule to be a transcriptional inhibitor of the QS-regulated operon vioABCDE. Docking calculations suggested a good correlation between binding affinity energies and inhibition effects, with all molecules positioned within the CviR autoinducer-binding domain (AIBD). The most active lactone yielded the best binding affinity energy, most probably due to its unprecedented binding with the AIBD. Our results show α-alkylidene δ-lactones as promising chemical scaffolds for the development of new QS inhibitors affecting LuxR/LuxI-systems. | pt_BR |
| dc.identifier.orcid | https://orcid.org/0000-0003-4284-9929 | pt_BR |
| dc.description.unidade | Instituto de Química (IQ) | pt_BR |
| dc.description.unidade | Faculdade UnB Ceilândia (FCE) | pt_BR |
| dc.description.unidade | Instituto de Ciências Biológicas (IB) | pt_BR |
| dc.description.unidade | Departamento de Biologia Celular (IB CEL) | pt_BR |
| Aparece en las colecciones: | Artigos publicados em periódicos e afins
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