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dc.contributor.authorDecloquement, Jennifer Nathalie Yolande-
dc.contributor.authorRamos Sobrinho, Roberto-
dc.contributor.authorElias, Samuel Galvão-
dc.contributor.authorBritto, Dahyana Santos-
dc.contributor.authorPuig, Alina Sandra-
dc.contributor.authorReis, Ailton-
dc.contributor.authorSilva, Rildo Alexandre Fernandes da-
dc.contributor.authorJúnior, Jaime Honorato-
dc.contributor.authorLuz, Edna Dora Martins Newman-
dc.contributor.authorPinho, Danilo Batista-
dc.contributor.authorMarelli, Jean Philippe-
dc.date.accessioned2023-10-05T21:07:49Z-
dc.date.available2023-10-05T21:07:49Z-
dc.date.issued2021-03-15-
dc.identifier.citationDECLOQUEMENT, Jennifer Nathalie Yolande et al. Phytophthora theobromicola sp. nov.: a new species causing black pod disease on cacao in Brazil. Frontiers in Microbiology, v. 12, 2021. DOI: https://doi.org/10.3389/fmicb.2021.537399. Disponível em: https://www.frontiersin.org/articles/10.3389/fmicb.2021.537399/full. Acesso em: 05 out. 2023.pt_BR
dc.identifier.urihttp://repositorio2.unb.br/jspui/handle/10482/46627-
dc.language.isoengpt_BR
dc.publisherFrontiers Media-
dc.rightsAcesso Abertopt_BR
dc.titlePhytophthora theobromicola sp. nov. : a new species causing black pod disease on cacao in Brazilpt_BR
dc.typeArtigopt_BR
dc.subject.keywordDiversidade biológicapt_BR
dc.subject.keywordCacaueiro - doenças e pragaspt_BR
dc.subject.keywordControle de pragaspt_BR
dc.subject.keywordPhytophthorapt_BR
dc.rights.licenseCopyright © 2021 Decloquement, Ramos-Sobrinho, Elias, Britto, Puig, Reis, da Silva, Honorato-Júnior, Luz, Pinho and Marelli. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Fonte: https://www.frontiersin.org/articles/10.3389/fmicb.2021.537399/full. Acesso em: 05 out. 2023.pt_BR
dc.identifier.doihttps://doi.org/10.3389/fmicb.2021.537399pt_BR
dc.description.abstract1Black pod disease, caused by Phytophthora species, is among the main limiting factors of cacao (Theobroma cacao L.) production. High incidence levels of black pod disease have been reported in Brazil, being induced by Phytophthora capsici, Phytophthora citrophthora, Phytophthora heveae, and Phytophthora palmivora. To assess the diversity of Phytophthora species affecting cacao in Brazil, 40 new isolates were obtained from cacao pods exhibiting symptoms of black pod disease collected in different smallholder farms in 2017. Further, ten cacao-infecting isolates morphologically identified as P. citrophthora and P. palmivora were molecularly characterized. The genomic regions beta-tubulin, elongation factor 1 alpha, heat shock protein 90, and internal transcribed spacer, and the mitochondrially encoded cytochrome c oxidase I and II genes were PCR-amplified and Sanger-sequenced from the cacao-infecting Phytophthora isolates. The morphological characterization and evaluation of the mycelial growth rates for the Phytophthora isolates were performed in vitro. Based on the molecular analysis and morphological comparisons, 19 isolates were identified as P. palmivora (clade 4). Interestingly, 31 isolates grouped together in the phylogenetic tree and were placed apart from previously known species in Phytophthora clade 2. Therefore, these isolates are considered as a new species herein referred to as Phytophthora theobromicola sp. nov., which produced papillate, semipapillate, and persistent sporangia on simple sporangiophores. The P. palmivora isolates were identified as A1 mating type by pairing each isolate with known A1 and A2 tester strains of P. capsici, but no oogonia/antheridia were observed when P. theobromicola was paired with the different tester strains. The P. theobromicola and P. citrophthora isolates showed higher mycelial growth rates, when compared to P. palmivora, on different media at 10, 15, and 20◦C, but similar values were observed when grown on clarified CA media at 25 and 30◦C. The pathogenicity tests carried out on pods of four cacao clones (CCN51, PS1319, Cepec2004, and CP49) showed significant variability among the isolates of both Phytophthora species, with P. theobromicola inducing higher rates of necrotic lesion expansion, when compared to P. palmivora. Here, two Phytophthora species were found associated with black pod disease in the state of Bahia, Brazil, and the previously undescribed P. theobromicola seems to be prevalent in field conditions. This is the first report of P. theobromicola on T. cacao. Also, these findings are crucial to improve the disease control strategies, and for the development of cacao materials genetically resistant to Phytophthora.pt_BR
dc.contributor.emaildanilopinho@unb.brpt_BR
dc.contributor.emailjean-philippe.marelli@effem.compt_BR
dc.contributor.affiliationUniversidade de Brasília, Departamento de Fitopatologiapt_BR
dc.contributor.affiliationBiophyto Plant Healthpt_BR
dc.contributor.affiliationUniversidade de Brasília, Departamento de Fitopatologiapt_BR
dc.contributor.affiliationMars Center for Cocoa Sciencept_BR
dc.contributor.affiliationU.S. Department of Agriculture, Agricultural Research Service, Subtropical Horticultural Research Stationpt_BR
dc.contributor.affiliationEmbrapa, Centro Nacional de Pesquisa de Hortaliçaspt_BR
dc.contributor.affiliationUniversidade de Brasília, Departamento de Fitopatologiapt_BR
dc.contributor.affiliationUniversidade Federal do Oeste da Bahia, Centro Multidisciplinar do Campus de Barrapt_BR
dc.contributor.affiliationCentro de Pesquisas do Cacau, Comissão Executiva do Plano da Lavoura Cacaueirapt_BR
dc.contributor.affiliationUniversidade de Brasília, Departamento de Fitopatologiapt_BR
dc.contributor.affiliationMars Plant Sciences Laboratorypt_BR
dc.description.unidadeInstituto de Ciências Biológicas (IB)pt_BR
dc.description.unidadeDepartamento de Fitopatologia (IB FIT)pt_BR
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