| Campo DC | Valor | Idioma |
|---|
| dc.contributor.author | Carvalho, Amandda Évelin Silva de | - |
| dc.contributor.author | Silva, Ingrid Gracielle Martins da | - |
| dc.contributor.author | Corrêa, José Raimundo | - |
| dc.contributor.author | Araujo, Felipe Saldanha de | - |
| dc.date.accessioned | 2023-09-25T19:38:20Z | - |
| dc.date.available | 2023-09-25T19:38:20Z | - |
| dc.date.issued | 2022-11-18 | - |
| dc.identifier.citation | CARVALHO, Amanda Évelin Silva de et. al. Regulatory T-Cell enhancement, expression of adhesion molecules, and production of anti-inflammatory factors are differentially modulated by spheroid-cultured mesenchymal stem cells. International Journal of Molecular Sciences, [s. l.], v. 23, 14349, 2022. DOI: https://doi.org/10.3390/ijms232214349. Disponível em: https://www.mdpi.com/1422-0067/23/22/14349. Acesso em: 25 set. 2023. | pt_BR |
| dc.identifier.uri | http://repositorio2.unb.br/jspui/handle/10482/46536 | - |
| dc.language.iso | eng | pt_BR |
| dc.publisher | MDPI | pt_BR |
| dc.rights | Acesso Aberto | pt_BR |
| dc.title | Regulatory T-Cell enhancement, expression of adhesion molecules, and production of anti-inflammatory factors are differentially modulated by spheroid-cultured mesenchymal stem cells | pt_BR |
| dc.type | Artigo | pt_BR |
| dc.subject.keyword | Células-tronco mesenquimais | pt_BR |
| dc.subject.keyword | Células T | pt_BR |
| dc.subject.keyword | Moléculas de adesão celular | pt_BR |
| dc.rights.license | (CC-BY) Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). | pt_BR |
| dc.identifier.doi | https://doi.org/10.3390/ijms232214349 | pt_BR |
| dc.description.abstract1 | The culture of mesenchymal stem cells (MSCs) as spheroids promotes a more physiological
cellular behavior, as it more accurately reflects the biological microenvironment. Nevertheless, mixed
results have been found regarding the immunosuppressive properties of spheroid-cultured MSCs
(3D-MSCs), the mechanisms of immunoregulation of 3D-MSCs being scarcely described at this point.
In the present study, we constructed spheroids from MSCs and compared their immunosuppressive
potential with that of MSCs cultured in monolayer (2D-MSCs). First, we evaluated the ability of
2D-MSCs and 3D-MSCs to control the activation and proliferation of T-cells. Next, we evaluated
the percentage of regulatory T-cells (Tregs) after the co-culturing of peripheral blood mononuclear
cells (PBMCs) with 2D-MSCs and 3D-MSCs. Finally, we investigated the expression of adhesion
molecules, as well as the expressions of several anti-inflammatory transcripts in 2D-MSCs and 3DMSCs maintained in both inflammatory and non-inflammatory conditions. Interestingly, our data
show that several anti-inflammatory genes are up-regulated in 3D-MSCs, and that these cells can
control T-cell proliferation. Nevertheless, 2D-MSCs are more efficient in suppressing the immune cell
proliferation. Importantly, contrary to what was observed in 3D-MSCs, the expressions of ICAM-1
and VCAM-1 are significantly upregulated in 2D-MSCs exposed to an inflammatory environment.
Furthermore, only 2D-MSCs are able to promote the enhancement of Tregs. Taken together, our
data clearly show that the immunosuppressive potential of MSCs is significantly impacted by
their shape, and highlights the important role of cell–cell adhesion molecules for optimal MSC
immunomodulatory function. | pt_BR |
| dc.identifier.orcid | https://orcid.org/0000-0001-7654-044X | pt_BR |
| dc.contributor.affiliation | University of Brasília, Hematology and Stem Cells Laboratory | pt_BR |
| dc.contributor.affiliation | University of Brasília, Molecular Pharmacology Laboratory | pt_BR |
| dc.contributor.affiliation | University of Brasília, Microscopy and Microanalysis Laboratory | pt_BR |
| dc.contributor.affiliation | University of Brasília, Microscopy and Microanalysis Laboratory | pt_BR |
| dc.contributor.affiliation | University of Brasília, Hematology and Stem Cells Laboratory | pt_BR |
| dc.description.unidade | Instituto de Ciências Biológicas (IB) | pt_BR |
| dc.description.unidade | Faculdade de Ciências da Saúde (FS) | pt_BR |
| dc.description.unidade | Departamento de Farmácia (FS FAR) | pt_BR |
| Aparece nas coleções: | Artigos publicados em periódicos e afins
|
