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dc.contributor.authorTeixeira, Antonio Raimundo Lima Cruz-
dc.contributor.authorGomes, Clever Cardoso-
dc.contributor.authorSá, Adriana Alves-
dc.contributor.authorNascimento, Rubens José-
dc.contributor.authorLauria-Pires, Liana-
dc.contributor.authorCastro, Ana Maria-
dc.contributor.authorBernal, Francisco Ernesto Moreno-
dc.contributor.authorSousa, Alessandro Oliveira de-
dc.date.accessioned2023-07-05T12:24:26Z-
dc.date.available2023-07-05T12:24:26Z-
dc.date.issued2023-
dc.identifier.citationTEIXEIRA, Antonio Raimundo Lima et al. Trypanosoma cruzi kDNA minicircle sequences integration into the vertebrate host genome: a biomarker for monitoring the efficacy of multidrug treatment of Chagas disease. International Journal of Scientific Research Updates, S.l., v. 5, n. 1, 170-207, 2023. DOI: https://doi.org/10.53430/ijsru.2023.5.1.0026. Disponível em: https://orionjournals.com/ijsru/content/trypanosoma-cruzi-kdna-minicircle-sequences-integration-vertebrate-host-genome-biomarker. Acesso em: 05 julho 2023.pt_BR
dc.identifier.urihttp://repositorio2.unb.br/jspui/handle/10482/46019-
dc.language.isoengpt_BR
dc.publisherOrion Journalspt_BR
dc.rightsAcesso Abertopt_BR
dc.titleTrypanosoma cruzi kDNA minicircle sequences integration into the vertebrate host genome : a biomarker for monitoring the efficacy of multidrug treatment of Chagas diseasept_BR
dc.typeArtigopt_BR
dc.subject.keywordTrypanosoma cruzipt_BR
dc.subject.keywordTransferência de kDNApt_BR
dc.subject.keywordBiomarcadorpt_BR
dc.subject.keywordTratamento com múltiplas drogaspt_BR
dc.subject.keywordChagas, Doença dept_BR
dc.rights.licenseCopyright © 2023 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0.pt_BR
dc.identifier.doihttps://doi.org/10.53430/ijsru.2023.5.1.0026pt_BR
dc.description.abstract1The protist Trypanosoma cruzi inserts kinetoplast DNA minicircle sequences into the host genome. Sensitive PCR with specific primer sets revealed protozoan nuclear DNA and kinetoplast DNA in agarose gels bands probed with radiolabel specific sequences for tissues of T. cruzi-infected rabbits and mice. Avian species are refractory to the infection, but chicks that hatched from T. cruzi-inoculated eggs retained the kinetoplast DNA in the embryonic germ line cells and developed parasite-free Chagas disease-like cardiomyopathy. A Target-primer TAIL-PCR with specific primer sets, Southern hybridization, cloning, and sequencing of the amplification products revealed kinetoplast minicircle sequences integration sites mainly in LINE-1 transposable elements and hitchhiking to several loci. This kinetoplast DNA biomarker was used to monitor the effect of multidrug treatment of T. cruzi-infected mice. A trypanocidal nitro heterocyclic compound in combination with an array of inhibitors of eukaryotic cell division prevented minicircle sequences transfer. Nine out of 12 inhibitors prevented the kinetoplast DNA integration into the macrophage genome. The multidrug treatment of T. cruzi-infected mice with benznidazole, azidothymidine and ofloxacin lowered the rate of minicircle sequences integrations into the mouse genome by 2.44-fold and reduced the rejection of the target heart cells.pt_BR
dc.contributor.affiliationUniversity of Brasília, Faculty of Medicine, Department of Pathologypt_BR
dc.contributor.affiliationFederal University of Goiás, Department of Histology, Embryology and Cell Biologypt_BR
dc.contributor.affiliationUniversity of Brasília, Faculty of Medicine, Department of Pathologypt_BR
dc.contributor.affiliationSambaia Regional Hospitalpt_BR
dc.contributor.affiliationUniversity of Brasília, Faculty of Medicine, Department of Pathologypt_BR
dc.contributor.affiliationMinistry of Science and Technology, National Technical Commission on Biosafetypt_BR
dc.contributor.affiliationUniversity of Brasília, Faculty of Medicine, Department of Pathologypt_BR
dc.contributor.affiliationCatholic University of Brasilia, Department of Medicinept_BR
dc.contributor.affiliationFederal University of Goiás, Institute for Tropical Pathology and Public Healthpt_BR
dc.contributor.affiliationUniversity of Brasília, Faculty of Agronomy and Veterinary Medicine, Animal Welfare and Avian Pathology Laboratorypt_BR
dc.contributor.affiliationUniversity of Brasília, Faculty of Medicine, Department of Pathologypt_BR
dc.contributor.affiliationLogistic Office for Health, Federal District Health Departmentpt_BR
dc.description.unidadeFaculdade de Medicina (FMD)-
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