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Universidade de Brasília
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dc.contributor.authorPittella-Silva, Fabio-
dc.contributor.authorYoon Ming Chin-
dc.contributor.authorHiu Ting Chan-
dc.contributor.authorNagayama, Satoshi-
dc.contributor.authorMiyauchi, Eisaku-
dc.contributor.authorLow, Siew-Kee-
dc.contributor.authorNakamura, Yusuke-
dc.date.accessioned2022-09-29T12:49:35Z-
dc.date.available2022-09-29T12:49:35Z-
dc.date.issued2020-06-09-
dc.identifier.citationPITTELLA-SILVA, Fabio et al. Plasma or serum: which Is preferable for mutation detection in liquid biopsy?. Clinical Chemistry, v. 66, n. 7, jul. 2020, p. 946-957, jul. 2020. DOI: https://doi.org/10.1093/clinchem/hvaa103.pt_BR
dc.identifier.urihttps://repositorio.unb.br/handle/10482/44933-
dc.language.isoInglêspt_BR
dc.publisherOxford University Presspt_BR
dc.rightsAcesso Restritopt_BR
dc.titlePlasma or serum : which Is preferable for mutation detection in liquid biopsy?pt_BR
dc.typeArtigopt_BR
dc.subject.keywordPlasmapt_BR
dc.subject.keywordSoropt_BR
dc.subject.keywordMutação genéticapt_BR
dc.subject.keywordBiópsia líquidapt_BR
dc.identifier.doihttps://doi.org/10.1093/clinchem/hvaa103pt_BR
dc.relation.publisherversionhttps://academic.oup.com/clinchem/article/66/7/946/5855218pt_BR
dc.description.abstract1Background Blood-based analysis of circulating tumor DNA (ctDNA) is a promising tool for cancer screening, monitoring relapse/recurrence and evaluating response to treatment. Although plasma is widely used to obtain ctDNA, biorepositories worldwide possess a huge number of serum samples and comparative studies on the use of serum vs plasma as ctDNA sources are essential. Methods We analyzed cell-free DNA (cfDNA) from matched EDTA-plasma and serum samples from healthy donors and patients with colorectal or lung cancer, and used targeted next-generation sequencing to evaluate mutation detection efficiency and reproducibility. Matched samples from healthy individuals were spiked with reference oligonucleotides and sequenced using the Ion-S5 Oncomine-Pan-Cancer panel. Detection efficiency in matched samples from patients with cancer was evaluated using 2 distinct gene panels and compared to mutations found in tissue-biopsy samples at diagnosis. Results Mean total cfDNA was 55% higher in serum samples and the presence of longer DNA fragments was significantly increased in serum compared with plasma samples (P = 0.0001 to 0.015). Spiked mutated nucleotides were detected in both samples, but allele frequencies (AF) were approximately half in serum compared with plasma, suggesting ctDNA from serum was more diluted by DNA of noncancerous origins. Matched samples from patients with cancer revealed that up to 44.8% of mutations with low AF were missed in serum samples and concordance rates with somatic mutations found in tissue biopsy at diagnosis was better in plasma samples. Conclusion The use of serum in retrospective studies should consider the limitations for detecting low AF mutations. Plasma is clearly preferable for prospective clinical applications of liquid biopsy.pt_BR
dc.contributor.affiliationUniversidade de Brasília, Faculdade de Ciências da Saúdept_BR
dc.contributor.affiliationCancer Precision Medicine Center, Japanese Foundation for Cancer Research, Tokyo, Japanpt_BR
dc.contributor.affiliationCancer Precision Medicine Center, Japanese Foundation for Cancer Research, Tokyo, Japanpt_BR
dc.contributor.affiliationDepartment of Gastroenterological Surgery, Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, Japanpt_BR
dc.contributor.affiliationDepartment of Respiratory Medicine, Tohoku University, Sendai, Japanpt_BR
dc.contributor.affiliationCancer Precision Medicine Center, Japanese Foundation for Cancer Research, Tokyo, Japanpt_BR
dc.contributor.affiliationCancer Precision Medicine Center, Japanese Foundation for Cancer Research, Tokyo, Japanpt_BR
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