Título:	SET domain-containing protein 4 (SETD4) is a newly identified cytosolic and nuclear Lysine Methyltransferase involved in breast cancer cell proliferation
Autor(es):	Faria, Jerusa Araújo Quintão Arantes Corrêa, Natássia Caroline Resende Andrade, Carolina de Campos, Ana Carolina de Angelis Almeida, Rubens dos Santos Samuel de Rodrigues, Thiago Souza Goes, Alfredo Miranda de Gomes, Dawidson Assis Pittella-Silva, Fabio
Afiliação do autor:	Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais (UFMG) Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais (UFMG) Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais (UFMG) Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais (UFMG) Universidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia Departamento de Informática, Centro Federal de Educação Tecnológica de Minas Gerais Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais (UFMG) Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais (UFMG) Universidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia
Assunto:	Mamas - câncer Proteínas
Data de publicação:	2013
Editora:	Hilaris
Referência:	FARIA, Jerusa Araújo Quintão Arantes et al. SET domain-containing protein 4 (SETD4) is a newly identified cytosolic and nuclear Lysine Methyltransferase involved in breast cancer cell proliferation. Journal of Cancer Science & Therapy, v. 5, n. 2, p. 59-65, 2013. DOI: 10.4172/1948-5956.1000185. Disponível em: https://www.hilarispublisher.com/open-access/set-domain-containing-protein-4-setd4-is-a-newly-identified-cytosolicand-nuclear-lysine-methyltransferase-involved-in-breast-cancer-cellproliferation-1948-5956.1000185.pdf.
Abstract:	Cancer is comprised of a multitude of epigenetic abnormalities, including the global loss and regional gain of DNA methylation as well as alterations in histone methylation. Here, we characterize a new methyltransferase, SET domain-containing protein 4 (SETD4), which is involved in breast carcinogenesis. Quantitative real-time PCR (qPCR) showed elevated expression levels of SETD4 in several breast cancer cell lines. SETD4 overexpression was confirmed by western blot analysis suggesting a correlation between high expression of SETD4 and a lack of the estrogen receptor (ER) in breast cancer. In addition, cell fractionation studies and confocal immunofluorescence revealed the nuclear and non-nuclear localization of this new protein. SETD4 knockdown in breast cancer cell lines significantly suppressed their proliferation and delayed the G1/S cell cycle transition without affecting apoptosis. Furthermore, western blot analysis showed that knockdown of SETD4 decreased cyclin D1 expression, revealing the involvement of SETD4 in cell cycle regulation. These data imply that SETD4 plays a crucial role in breast carcinogenesis and could be a novel molecular target for the development of new strategies for the diagnosis and treatment of breast cancer.
Licença:	Copyright: © 2013 Arantes Faria JAQ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI:	10.4172/1948-5956.1000185
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