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dc.contributor.authorGonçalves, Luiz Ricardo-
dc.contributor.authorPaludo, Giane Regina-
dc.contributor.authorBisol, Talita Barcelos-
dc.contributor.authorPerles, Lívia-
dc.contributor.authorOliveira, Laryssa Borges de-
dc.contributor.authorOliveira, Camila Manoel de-
dc.contributor.authorSilva, Thiago Merighi Vieira da-
dc.contributor.authorNantes, Wesley Arruda Gimenes-
dc.contributor.authorDuarte, Matheus Almeida-
dc.contributor.authorSantos, Filipe Martins-
dc.contributor.authorPorfírio, Grasiela Edith de Oliveira-
dc.contributor.authorHirano, Líria Queiroz Luz-
dc.contributor.authorHerrera, Heitor Miraglia-
dc.contributor.authorBattesti, Darci Moraes Barros-
dc.contributor.authorMachado, Rosangela Zacarias-
dc.contributor.authorAndré, Marcos Rogério-
dc.date.accessioned2022-05-19T15:45:08Z-
dc.date.available2022-05-19T15:45:08Z-
dc.date.issued2021-
dc.identifier.citationGONÇALVES, Luiz Ricardo et al. Molecular detection of piroplasmids in synanthropic rodents, marsupials, and associated ticks from Brazil, with phylogenetic inference of a putative novel Babesia sp. from white-eared opossum (Didelphis albiventris). Parasitology Research, v. 120, n. 10, p. 3537-3546, 2021. DOI: https://doi.org/10.1007/s00436-021-07284-8. Disponível em: https://link.springer.com/article/10.1007/s00436-021-07284-8. Acesso em: 19 maio 2022.pt_BR
dc.identifier.urihttps://repositorio.unb.br/handle/10482/43750-
dc.language.isoInglêspt_BR
dc.publisherSpringer Naturept_BR
dc.rightsAcesso Restritopt_BR
dc.titleMolecular detection of piroplasmids in synanthropic rodents, marsupials, and associated ticks from Brazil, with phylogenetic inference of a putative novel Babesia sp. from white-eared opossum (Didelphis albiventris)pt_BR
dc.typeArtigopt_BR
dc.subject.keywordCapivarapt_BR
dc.subject.keywordGambápt_BR
dc.subject.keywordPiroplasmídeospt_BR
dc.rights.license© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021pt_BR
dc.identifier.doihttps://doi.org/10.1007/s00436-021-07284-8pt_BR
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s00436-021-07284-8pt_BR
dc.description.abstract1The order Piroplasmida encompasses tick-borne pathogens of veterinary and medical importance positioned in two main families: Babesiidae and Theileriidae. Even though previous studies carried out in Brazil recorded the occurrence of piroplasmid species circulating in small mammals, 18S RNA gene sequences were only partially sequenced, preventing the assessment of their phylogenetic positioning. The current study aimed to detect and characterize, using morphological, molecular, and bioinformatic approaches, piroplasmids from wild mammals and associated ticks sampled in Central-Western Brazil. Out of 67 Didelphis albiventris sampled, 22 (16.4%) were positive for piroplasmids by PCR. In contrast, none of the 48 small rodents and 14 capybaras (Hydrochoerus hydrochaeris) was PCR-positive. Four Amblyomma dubitatum ticks—one from Rattus rattus, one from H. hydrochaeris, and two from D. albiventris—out of 114 Amblyomma spp. DNA samples were positive for piroplasmids by PCR. The phylogenetic inference performed using the near-complete 18S rRNA gene positioned the putative novel piroplasmid species detected in D. albiventris and associated A. dubitatum ticks near to Babesia sensu lato clade (Western group—cluster III) and distant from the Australian marsupial-associated piroplasms. Phylogenetic inferences based on two additional molecular markers, namely hsp-70 and cox-1, supported the near-complete 18S rRNA gene phylogenetic inference. Finally, the partial 18S rRNA gene sequences detected in ticks from rodents (R. rattus and H. hydrochaeris) showed 97.2–99.4% identity with the Piroplasmida previously detected in a capybara from Brazil, raising evidence that a still uncharacterized piroplasmid species has been identified in the capybara, the largest rodent species from South America.pt_BR
dc.description.unidadeFaculdade de Agronomia e Medicina Veterinária (FAV)-
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