Multiple LEDT wavelengths modulate the Akt signaling pathways and attenuate pathological events in mdx dystrophic muscle cells

Rocha, Guilherme Luiz da; Mizobuti, Daniela Sayuri; Silva, Heloina Nathalliê Mariano da; Covatti, Caroline; Lourenço, Caroline Caramano de; Salvador, Marcos José; Pereira, Elaine Cristina Leite; Minatel, Elaine

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Campo DC	Valor	Lengua/Idioma
dc.contributor.author	Rocha, Guilherme Luiz da	-
dc.contributor.author	Mizobuti, Daniela Sayuri	-
dc.contributor.author	Silva, Heloina Nathalliê Mariano da	-
dc.contributor.author	Covatti, Caroline	-
dc.contributor.author	Lourenço, Caroline Caramano de	-
dc.contributor.author	Salvador, Marcos José	-
dc.contributor.author	Pereira, Elaine Cristina Leite	-
dc.contributor.author	Minatel, Elaine	-
dc.date.accessioned	2022-04-20T13:53:48Z	-
dc.date.available	2022-04-20T13:53:48Z	-
dc.date.issued	2022-04-05	-
dc.identifier.citation	ROCHA, Guilherme Luiz da et al. Multiple LEDT wavelengths modulate the Akt signaling pathways and attenuate pathological events in mdx dystrophic muscle cells. Photochemical & Photobiological Sciences, [S.l], 2022. DOI: https://doi.org/10.1007/s43630-022-00216-0.	pt_BR
dc.identifier.uri	https://repositorio.unb.br/handle/10482/43511	-
dc.language.iso	Inglês	pt_BR
dc.publisher	Springer	pt_BR
dc.rights	Acesso Restrito	pt_BR
dc.title	Multiple LEDT wavelengths modulate the Akt signaling pathways and attenuate pathological events in mdx dystrophic muscle cells	pt_BR
dc.type	Artigo	pt_BR
dc.subject.keyword	Fotobiomodulação	pt_BR
dc.subject.keyword	Inflamação	pt_BR
dc.identifier.doi	https://doi.org/10.1007/s43630-022-00216-0	pt_BR
dc.relation.publisherversion	https://link.springer.com/article/10.1007/s43630-022-00216-0	pt_BR
dc.description.abstract1	This study is aimed at investigating the effects of LEDT, at multiple wavelengths, on intracellular calcium concentration; on transient receptor potential canonical channels; on calcium-binding protein; on myogenic factors; on myosin heavy chains; on Akt signaling pathway; on inflammatory markers; and on the angiogenic-inducing factor in dystrophic muscle cell culture experimental model. Dystrophic primary muscle cells were submitted to LEDT, at multiple wavelengths (420 nm, 470 nm, 660 nm, and 850 nm), and evaluated after 48 h for cytotoxic effects and intracellular calcium content. TRPC-1, TRPC-6, Calsequestrin, MyoD, Myogenin, MHC-slow, MHC-fast, p-AKT, p-mTOR, p-FoxO1, Myostatin, NF-κB, TNF-α, and VEGF levels were evaluated in dystrophic primary muscle cells by western blotting. The LEDT, at multiple wavelengths, treated-mdx muscle cells showed no cytotoxic effect and significant lower levels in [Ca2 +]i. The mdx muscle cells treated with LEDT showed a significant reduction of TRPC-1, NF-κB, TNF-α and MyoD levels and a significant increase of Myogenin, MHC-slow, p-AKT, p-mTOR, p-FoxO1 levels, and VEGF levels. Our findings suggest that different LEDT wavelengths modulate the Akt-signaling pathways and attenuate pathological events in dystrophic muscle cells, and a combined multiwavelength irradiation protocol may even provide a potentially therapeutic strategy for muscular dystrophies.	pt_BR
dc.description.unidade	Faculdade UnB Ceilândia (FCE)	pt_BR
dc.description.ppg	Programa de Pós-Graduação em Ciências da Reabilitação	pt_BR
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