Paracoccidioides HSP90 can be found in the cell surface and is a target for antibodies with therapeutic potential

Moura, Ágata Nogueira D’Áurea; Oliveira, Diane Sthefany Lima de; Paredes, Verenice; Rocha, Letícia Barboza; Oliveira, Fabiana Freire Mende de; Lessa, Gustavo Meirelles; Riasco Palacios, Juan Fernando; Casadevall, Arturo; Albuquerque, Patrícia; Felipe, Maria Sueli Soares; Piazza, Roxane Maria Fontes; Nicola, André Moraes

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Campo DC	Valor	Idioma
dc.contributor.author	Moura, Ágata Nogueira D’Áurea	-
dc.contributor.author	Oliveira, Diane Sthefany Lima de	-
dc.contributor.author	Paredes, Verenice	-
dc.contributor.author	Rocha, Letícia Barboza	-
dc.contributor.author	Oliveira, Fabiana Freire Mende de	-
dc.contributor.author	Lessa, Gustavo Meirelles	-
dc.contributor.author	Riasco Palacios, Juan Fernando	-
dc.contributor.author	Casadevall, Arturo	-
dc.contributor.author	Albuquerque, Patrícia	-
dc.contributor.author	Felipe, Maria Sueli Soares	-
dc.contributor.author	Piazza, Roxane Maria Fontes	-
dc.contributor.author	Nicola, André Moraes	-
dc.date.accessioned	2022-04-14T12:20:21Z	-
dc.date.available	2022-04-14T12:20:21Z	-
dc.date.issued	2020-09-28	-
dc.identifier.citation	MOURA, Ágata Nogueira D’Áurea et al. Paracoccidioides HSP90 can be found in the cell surface and is a target for antibodies with therapeutic potential. Journal Fungi, v. 6, n. 4, 193, 2020. DOI: https://doi.org/10.3390/jof6040193. Disponível em: https://www.mdpi.com/2309-608X/6/4/193. Acesso em: 14 abr. 2022.	pt_BR
dc.identifier.uri	https://repositorio.unb.br/handle/10482/43452	-
dc.language.iso	Inglês	pt_BR
dc.publisher	MDPI	pt_BR
dc.rights	Acesso Aberto	pt_BR
dc.title	Paracoccidioides HSP90 can be found in the cell surface and is a target for antibodies with therapeutic potential	pt_BR
dc.type	Artigo	pt_BR
dc.subject.keyword	Anticorpos	pt_BR
dc.subject.keyword	Micoses	pt_BR
dc.subject.keyword	Infecção - fungos	pt_BR
dc.subject.keyword	Tratamento	pt_BR
dc.identifier.doi	https://doi.org/10.3390/jof6040193	pt_BR
dc.description.abstract1	Paracoccidioidomycosis (PCM) is one of the most frequent systemic mycoses in Latin America. It affects mainly male rural workers in impoverished regions, and the therapy can last up to two years or use drugs that are very toxic. Given the need for novel safe and effective approaches to treat PCM, we have been developing monoclonal antibodies (mAbs) that could be used not only to block specific fungal targets, but also modulate the host’s antifungal immunity. In this work we show the generation of and promising results with an mAb against Heat Shock Protein (HSP)90, a molecular chaperone that is an important virulence factor in fungi. Using recombinant Paracoccidioides lutzii (Pb01) and P. brasiliensis (Pb18) HSP90 proteins produced in E. coli, we immunized mice and generated polyclonal antibodies and an IgG1 hybridoma mAb. The proteins were very immunogenic and both the polyclonal serum and mAb were used in immunofluorescence experiments, which showed binding of antibodies to the yeast cell surface. The mAb successfully opsonized P. lutzii and P. brasiliensis cells in co-incubations with J774.16 macrophage-like cells. Our results suggest that this mAb could serve as the basis for new immunotherapy regimens for PCM.	pt_BR
dc.identifier.orcid	https://orcid.org/ 0000-0003-0480-5028	pt_BR
dc.identifier.orcid	https://orcid.org/ 0000-0002-2655-4672	pt_BR
dc.identifier.orcid	https://orcid.org/ 0000-0001-7832-058X	pt_BR
dc.identifier.orcid	https://orcid.org/ 0000-0002-9170-1034	pt_BR
dc.identifier.orcid	https://orcid.org/ 0000-0002-9402-9167	pt_BR
dc.identifier.orcid	https://orcid.org/ 0000-0001-8160-7784	pt_BR
dc.identifier.orcid	https://orcid.org/ 0000-0001-8656-5835	pt_BR
dc.description.unidade	Faculdade de Medicina (FMD)	-
Aparece nas coleções:	Artigos publicados em periódicos e afins UnB - Professores Eméritos