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dc.contributor.authorGraziane, Daniel-
dc.contributor.authorRibeiro, J. V. V.-
dc.contributor.authorCruz, V. S.-
dc.contributor.authorGomes, R. M.-
dc.contributor.authorAraujo, E. G.-
dc.contributor.authorSantos Júnior, Agenor de Castro Moreira dos-
dc.contributor.authorTomaz, H. C. M.-
dc.contributor.authorCastro, C. H.-
dc.contributor.authorFontes, Wagner-
dc.contributor.authorBatista, K. A.-
dc.contributor.authorFernandes, K. F.-
dc.contributor.authorXavier, C. H.-
dc.date.accessioned2021-08-14T13:42:12Z-
dc.date.available2021-08-14T13:42:12Z-
dc.date.issued2021-
dc.identifier.citationGRAZIANI, D. et al. Oxidonitrergic and antioxidant effects of a low molecular weight peptide fraction from hardened bean (Phaseolus vulgaris) on endothelium. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 54, n. 6, e10423, 2021. DOI: https://doi.org/10.1590/1414-431x202010423. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000600602&lng=en&nrm=iso. Acesso em: 13 maio 2021.pt_BR
dc.identifier.urihttps://repositorio.unb.br/handle/10482/41708-
dc.language.isoInglêspt_BR
dc.publisherAssociação Brasileira de Divulgação Científicapt_BR
dc.rightsAcesso Abertopt_BR
dc.titleOxidonitrergic and antioxidant effects of a low molecular weight peptide fraction from hardened bean (Phaseolus vulgaris) on endotheliumpt_BR
dc.typeArtigopt_BR
dc.subject.keywordFeijãopt_BR
dc.subject.keywordPeptídeospt_BR
dc.subject.keywordStress oxidativopt_BR
dc.subject.keywordÓxido nítricopt_BR
dc.rights.license(CC BY)pt_BR
dc.identifier.doihttps://doi.org/10.1590/1414-431X202010423pt_BR
dc.description.abstract1About 3000 tons of beans are not used in human food due to hardening. Several studies on bean-derived bioactive peptides have shown potential to treat some diseases, including those relying on oxidative dysfunctions. We assessed the effects of peptides extracted from hardened bean Phaseolus vulgaris (PV) on reactive oxygen species (ROS) and nitric oxide (NO) production, cytotoxic and cytoprotective effects in endothelial cells, and oxidonitrergic-dependent vasodilating effects. Extract was composed by peptide fraction <3 kDa (PV3) from hardened common bean residue. PV3 sequences were obtained and analyzed with bioinformatics. Human umbilical vein endothelial cells were treated with 10, 20, 30, and 250 µg/mL PV3. Oxidative stress was provoked by 3% H2O2. Cytotoxicity and cytoprotective effects were evaluated by MTT assay, whereas, ROS and NO were quantified using DHE and DAF-FM fluorescent probes by confocal microscopy. NO- and endothelium-dependent vasodilating effects of PV3 were assessed in isolated aortic rings. We found 35 peptides with an average mass of 1.14 kDa. There were no cell deaths with 10 and 20 μg/mL PV3. PV3 at 30 μg/mL increased cell viability, while cytotoxicity was observed only with 250 μg/mL PV3. PV3 at 10 μg/mL was able to protect cells from oxidative stress. PV3 also increased NO release without causing cell death. It also reduced relative ROS production induced by H2O2. PV3 vasodilating effects relied on endothelium-dependent NO release. PV3 obtained from low-commercial-value bean displays little cytotoxicity and exerts antioxidant effects, whereas it increases endothelial NO release.pt_BR
dc.identifier.orcidhttp://orcid.org/0000-0002-4997-2658pt_BR
dc.identifier.orcidhttp://orcid.org/0000-0003-3937-1071pt_BR
dc.identifier.orcidhttp://orcid.org/0000-0002-8914-5964pt_BR
dc.identifier.orcidhttp://orcid.org/0000-0002-9012-3287pt_BR
dc.identifier.orcidhttp://orcid.org/0000-0002-0242-2827pt_BR
dc.identifier.orcidhttp://orcid.org/0000-0001-8485-2603pt_BR
dc.identifier.orcidhttp://orcid.org/0000-0003-2079-2140pt_BR
dc.identifier.orcidhttp://orcid.org/0000-0002-9510-1124pt_BR
dc.identifier.orcidhttp://orcid.org/0000-0001-5140-8573pt_BR
dc.identifier.orcidhttp://orcid.org/0000-0003-4396-032Xpt_BR
dc.identifier.orcidhttp://orcid.org/0000-0002-6558-5447pt_BR
dc.identifier.orcidhttp://orcid.org/0000-0003-4006-8213pt_BR
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