Isolation, identification, and screening of lactic acid bacteria with probiotic potential in silage of different species of forage plants, cocoa beans, and artisanal salami

Leandro, Eliana dos Santos; Ginani, Verônica Cortez; Alencar, Ernandes Rodrigues de; Pereira, Odilon Gomes; Rose, Ester Cardoso Paes; Vale, Helson Mário Martins do; Pratesi, Riccardo; Hecht, Mariana Machado; Cavalcanti, Marilia Hermes; Tavares, Caroline Stéfany Oliveira

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Campo DC	Valor	Idioma
dc.contributor.author	Leandro, Eliana dos Santos	-
dc.contributor.author	Ginani, Verônica Cortez	-
dc.contributor.author	Alencar, Ernandes Rodrigues de	-
dc.contributor.author	Pereira, Odilon Gomes	-
dc.contributor.author	Rose, Ester Cardoso Paes	-
dc.contributor.author	Vale, Helson Mário Martins do	-
dc.contributor.author	Pratesi, Riccardo	-
dc.contributor.author	Hecht, Mariana Machado	-
dc.contributor.author	Cavalcanti, Marilia Hermes	-
dc.contributor.author	Tavares, Caroline Stéfany Oliveira	-
dc.date.accessioned	2021-07-28T12:35:36Z	-
dc.date.available	2021-07-28T12:35:36Z	-
dc.date.issued	2021	-
dc.identifier.citation	LEANDRO, Eliana dos Santos et al. Isolation, identification, and screening of lactic acid bacteria with probiotic potential in silage of different species of forage plants, cocoa beans, and artisanal salami. Probiotics and Antimicrobial Proteins, v. 13, p. 173–186, 2021. DOI: https://doi.org/10.1007/s12602-020-09679-y.	pt_BR
dc.identifier.uri	https://repositorio.unb.br/handle/10482/41513	-
dc.language.iso	Inglês	pt_BR
dc.publisher	Springer	pt_BR
dc.rights	Acesso Restrito	pt_BR
dc.title	Isolation, identification, and screening of lactic acid bacteria with probiotic potential in silage of different species of forage plants, cocoa beans, and artisanal salami	pt_BR
dc.type	Artigo	pt_BR
dc.subject.keyword	Bactérias lácticas	pt_BR
dc.subject.keyword	Probióticos	pt_BR
dc.subject.keyword	Fermentação	pt_BR
dc.identifier.doi	https://doi.org/10.1007/s12602-020-09679-y	pt_BR
dc.relation.publisherversion	https://link.springer.com/article/10.1007/s12602-020-09679-y	pt_BR
dc.description.abstract1	The objective of this study was to isolate and characterize lactic acid bacteria with probiotic potential in silages of different species of forage plants, cocoa beans, and artisanal salami. The obtained isolates were submitted to the following evaluations: (i) screening for tolerance to pH 2 and bile salts, (ii) genotypic identification of isolates, (iii) survival in simulated gastric and pancreatic conditions, (iv) antimicrobial activity, (v) antibiotic susceptibility and safety, and (vi) properties associated with adhesion capacity. A total of 82 isolates were obtained and were screened for pH 2.0 tolerance and capacity to growth in the presence of bile salts (1.0 and 2.0%). Only 19 strains simultaneously presented tolerance to pH 2.0 and bile salts. These 19 strains were evaluated for genetic profile by Box-PCR. Subsequently, the selected strains were subjected to partial sequencing of the 16S rRNA gene. The species Lactobacillus plantarum was prevalent. The identified strains were evaluated for survival under simulated gastric and pancreatic conditions. Some strains have shown tolerance in both conditions. Different strains showed variations in antimicrobial activity, susceptibility to antibiotics, and properties associated with adhesion (hydrophobicity, autoaggregation, coaggregation, and adhesion to CaCo2 cells). All strains were negative for hemolysis, DNase, gelatinase, and biogenic amine synthesis activity. The L. plantarum SBR64.7 strain can be considered the most promising for it presented the lowest viability reduction when exposed to gastric and pancreatic juices.	pt_BR
dc.identifier.orcid	https://orcid.org/0000-0003-3120-5925	pt_BR
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