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Universidade de Brasília
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dc.contributor.authorMoura, Ágata Nogueira D’Áurea-
dc.contributor.authorOliveira, Diane Sthefany Lima de-
dc.contributor.authorParedes, Verenice-
dc.contributor.authorRocha, Leticia Barboza-
dc.contributor.authorOliveira, Fabiana Freire Mendes de-
dc.contributor.authorLessa, Gustavo Meirelles-
dc.contributor.authorRiasco Palacios, Juan Fernando-
dc.contributor.authorCasadevall, Arturo-
dc.contributor.authorAlbuquerque, Patrícia-
dc.contributor.authorFelipe, Maria Sueli Soares-
dc.contributor.authorPiazza, Roxane Maria Fontes-
dc.contributor.authorNicola, André Moraes-
dc.date.accessioned2021-05-19T13:57:44Z-
dc.date.available2021-05-19T13:57:44Z-
dc.date.issued2020-
dc.identifier.citationMOURA, Ágata Nogueira D’Áurea. Paracoccidioides HSP90 can be found in the cell surface and is a target for antibodies with therapeutic potential. Journal of Fungi, v. 6, n. 4, 193, 2020. DOI: https://doi.org/10.3390/jof6040193. Disponível em: https://www.mdpi.com/2309-608X/6/4/193. Acesso em: 19 maio 2021.pt_BR
dc.identifier.urihttps://repositorio.unb.br/handle/10482/40940-
dc.language.isoInglêspt_BR
dc.publisherMDPIpt_BR
dc.rightsAcesso Abertopt_BR
dc.titleParacoccidioides HSP90 can be found in the cell surface and is a target for antibodies with therapeutic potentialpt_BR
dc.typeArtigopt_BR
dc.subject.keywordAnticorpos monoclonaispt_BR
dc.subject.keywordParacoccidioidespt_BR
dc.subject.keywordParacoccidioidomicosept_BR
dc.subject.keywordFungospt_BR
dc.rights.licenseCopyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).pt_BR
dc.identifier.doihttps://doi.org/10.3390/jof6040193pt_BR
dc.description.abstract1Paracoccidioidomycosis (PCM) is one of the most frequent systemic mycoses in Latin America. It affects mainly male rural workers in impoverished regions, and the therapy can last up to two years or use drugs that are very toxic. Given the need for novel safe and effective approaches to treat PCM, we have been developing monoclonal antibodies (mAbs) that could be used not only to block specific fungal targets, but also modulate the host’s antifungal immunity. In this work we show the generation of and promising results with an mAb against Heat Shock Protein (HSP)90, a molecular chaperone that is an important virulence factor in fungi. Using recombinant Paracoccidioides lutzii (Pb01) and P. brasiliensis (Pb18) HSP90 proteins produced in E. coli, we immunized mice and generated polyclonal antibodies and an IgG1 hybridoma mAb. The proteins were very immunogenic and both the polyclonal serum and mAb were used in immunofluorescence experiments, which showed binding of antibodies to the yeast cell surface. The mAb successfully opsonized P. lutzii and P. brasiliensis cells in co-incubations with J774.16 macrophage-like cells. Our results suggest that this mAb could serve as the basis for new immunotherapy regimens for PCM.pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-0480-5028pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-2655-4672pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-7832-058Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-9170-1034pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-9402-9167pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8160-7784pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8656-5835pt_BR
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