Título:	Bioprospecting microbial diversity for lignin valorization : dry and wet screening methods
Autor(es):	Gonçalves, Carolyne Caetano Bruce, Thiago Silva, Caio de Oliveira Gorgulho Ferreira Fillho, Edivaldo Ximenes Noronha, Eliane Ferreira Carlquist, Magnus Parachin, Nádia Skorupa
Assunto:	Enzimas Lignina Biossensores
Data de publicação:	9-Jun-2020
Editora:	Frontiers
Referência:	GONÇALVES, Carolyne Caetano et al. Bioprospecting microbial diversity for lignin valorization: dry and wet screening methods. Frontiers in Microbiology, v. 11, art. 1081, 09 jun. 2020. DOI:https://doi.org/10.3389/fmicb.2020.01081. Disponível em: https://www.frontiersin.org/articles/10.3389/fmicb.2020.01081/full. Acesso em: 18 fev. 2021.
Abstract:	Lignin is an abundant cell wall component, and it has been used mainly for generating steam and electricity. Nevertheless, lignin valorization, i.e. the conversion of lignin into high value-added fuels, chemicals, or materials, is crucial for the full implementation of cost-effective lignocellulosic biorefineries. From this perspective, rapid screening methods are crucial for time- and resource-efficient development of novel microbial strains and enzymes with applications in the lignin biorefinery. The present review gives an overview of recent developments and applications of a vast arsenal of activity and sequence-based methodologies for uncovering novel microbial strains with ligninolytic potential, novel enzymes for lignin depolymerization and for unraveling the main metabolic routes during growth on lignin. Finally, perspectives on the use of each of the presented methods and their respective advantages and disadvantages are discussed.
Licença:	Copyright © 2020 Gonçalves, Bruce, Silva, Fillho, Noronha, Carlquist and Parachin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms
DOI:	https://doi.org/10.3389/fmicb.2020.01081
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