Título:	In silico identification and characterization of novel microsatellite loci for the Blue-and-yellow Macaw Ara ararauna (Linnaeus, 1758) (Psittaciformes, Psittacidae)
Autor(es):	Lima-Rezende, Cássia Alves Fernandes, Gislaine Aparecida Silva, Helder Elias da Dobkowski-Marinho, Sarah Santos, Victor Fernandes Rodrigues, Fernando Pacheco Caparroz, Renato
ORCID:	http://orcid.org/0000-0003-4883-4023
Assunto:	Animais selvagens em cativeiro Tráfico de animais Parentesco Papagaio (Ave) Vida selvagem - conservação
Data de publicação:	2019
Editora:	Sociedade Brasileira de Genética
Referência:	LIMA-REZENDE, Cássia Alves et al. In silico identification and characterization of novel microsatellite loci for the Blue-and-yellow Macaw Ara ararauna (Linnaeus, 1758) (Psittaciformes, Psittacidae). Genetics and Molecular Biology, v. 42, n. 1, p. 68-73, 2019. DOI: https://doi.org/10.1590/1678-4685-gmb-2017-0338. Disponível em: http://scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100068. Acesso em: 23 jan. 2020.
Abstract:	The illegal trade is a major threat to many bird species, and parrots are common victims of this activity. Domestic and international pet markets are interested on different parrot species, such as the Blue-and-yellow Macaw (Ara ararauna). This South American macaw is not globally threatened, but is under protection from over-exploitation. This study aimed to identify and characterize novel microsatellite loci for population and parentage analysis of A. ararauna. Scaffold sequences of Ara macao available in the NCBI database were used for microsatellite searches using MsatCommander software. We tested a total of 28 loci, from which 25 were polymorphic, one was monomorphic, and two did not generated amplification products. For polymorphic loci, the mean number of alleles was 8.24 (4 – 15 alleles per locus), the observed heterozygosity ranged from 0.333 to 0.917, and the expected heterozygosity from 0.353 to 0.890. The paternity exclusion probability and identity probability were highly discriminatory. Thus, these novel microsatellite markers can be useful for population assignment and paternity tests, helping the authorities to manage macaws from the illegal trafficking and control commercial breeders.
Licença:	License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License (type CC-BY), which permits unrestricted use, distribution and reproduction in any medium, provided the original article is properly cited.
DOI:	https://doi.org/10.1590/1678-4685-gmb-2017-0338
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