Título:	Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum
Autor(es):	Costa, Gabriella Gusmão Pereira da Silva, Cristian Aldemar Gasca Gomes, João Victor Dutra Torres, Amanda Gomes Santos, Izulmé Rita Imaculada Almeida, Fabrício Tavares Cunha de Fagg, Christopher William Simeoni, Luiz Alberto Silveira, Dâmaris Gomes-Copeland, Kicia Karinne Pereira
ORCID:	http://orcid.org/0000-0002-3393-5609 http://orcid.org/0000-0003-4392-4230 http://orcid.org/0000-0001-6098-3852 http://orcid.org/0000-0001-9599-4578 http://orcid.org/0000-0002-1503-1497 http://orcid.org/0000-0002-0531-7483 http://orcid.org/0000-0001-6072-7832 http://orcid.org/0000-0003-3401-290X http://orcid.org/0000-0003-1851-5224 http://orcid.org/0000-0003-3539-2916
Assunto:	Alcalóides Biossíntese Cromatografia a líquido Licorina Espectrometria de massa
Data de publicação:	2019
Editora:	Sociedade Brasileira de Farmacognosia
Referência:	COSTA, Gabriella Gusmão Pereira da et al. Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum. Revista Brasileira de Farmacognosia, v. 29, n. 2, p. 262-265, 2019. DOI: https://doi.org/10.1016/j.bjp.2019.02.001. Disponível em: http://scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2019000200262. Acesso em: 23 jan.2020.
Abstract:	Hippeastrum goianum (Ravenna) Meerow, Amaryllidaceae, is an endemic species from the Cerrado, Brazil; there are only few studies about its chemistry or biological activity. This study aimed to investigate the occurrence of lycorine in extracts from in vitro H. goianum plantlets, as well as evaluate a possible inhibition of acetylcholinesterase. The ethanol extract of plantlets produced by in vitro seed germination and micropropagation of bulblets was obtained from seedlings from in vitro germination, while the ethanol extract micropropagtion of bulblets was obtained from a subculture of those seedlings. The presence of lycorine was detected in only in the micropropagation of bulblets. The micropropagation of bulblets was more active than the plantlets produced by in vitro seed germination, with an IC50 of 114.8 ± 0.95 µg/ml and IC50 386.00 ± 0.97 µg/ml, respectively. These results showed that both in vitro germination and micropropagation of H. goianum can lead to the biosynthesis of lycorine. Moreover, the micropropagation led to improved anticholinesterase activity.
Licença:	This is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/licenses/by-nc-nd/4.0/).
DOI:	https://doi.org/10.1016/j.bjp.2019.02.001
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