Título:	Mesenchymal stem cells in osteotomy repair after tibial tuberosity advancement in dogs with cranial cruciate ligament injury
Autor(es):	Santos, Clarissa Rocha dos Filgueiras, Richard da Rocha Malard, Patrícia Furtado Vianna, Andre Rodrigues da Cunha Barreto Nogueira, Kaique Leite, Carolina da Silva Lima, Eduardo Maurício Mendes de
Assunto:	Ortopedia Biotecnologia Articulações Joelhos
Data de publicação:	14-Jun-2018
Editora:	Springer Open
Referência:	SANTOS, Clarissa Rocha dos et al. Mesenchymal stem cells in osteotomy repair after tibial tuberosity advancement in dogs with cranial cruciate ligament injury. Journal of Experimental Orthopaedics, v. 5, n. 17, 2018. DOI: https://doi.org/10.1186/s40634-018-0130-z. Disponível em: https://jeo-esska.springeropen.com/articles/10.1186/s40634-018-0130-z.
Abstract:	Background: The cranial cruciate ligament rupture (CCLR) is the most commonly encountered orthopedic condition in dogs. Among the various techniques to treat this condition, tibial tuberosity advancement (TTA) has been used to obtain rapid recovery of the affected knee. The objective of this study was to evaluate the viability of the use of mesenchymal stem cells (MSC) implanted in the osteotomy site obtained by TTA in nine dogs diagnosed with CCLR. Methods: The MSC were isolated from the adipose tissue of the dogs and cultured for eight days, the animals were divided into two groups. Animals from the treated group (GT) received cell transport medium containing about 1.5 millions MSC, and the animals from the control group (GC) received only the cell transport medium. The study was performed in a double-blind manner using radiographs acquired on days 15, 30, 60 and 120 after the procedure. Evaluations of the density of the trabecular bone were performed using image analysis software. The results were subjected to descriptive statistical analysis, followed by the normality test, Chi-square test, Mann-Whitney test and Tukey’s multiple comparison test for p ≤ 0.05. Results: After 30 days of the procedure, the animals of the GT presented an ossification mean 36.45% greater (p ≤ 0.033) than the GC, and there were no statistical differences for the other periods. Conclusions: Despite the total bone ossification within the expected period, there was no minimization of the estimated recovery time with the application of MSC, and inflammatory factors should be considered for reassessment of the therapeutic intervention time.
Unidade Acadêmica:	Faculdade de Agronomia e Medicina Veterinária (FAV)
Licença:	© The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
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