Título:	Omega-3 docosahexaenoic acid induces pyroptosis cell death in triple-negative breast cancer cells
Autor(es):	Pizato, Nathalia Marcolini Pelucio Luzete, Beatriz Christina Kiffer, Larissa Fernanda Melo Vasconcelos Corrêa, Luís Henrique Santos, Igor de Oliveira Assumpção, José Antônio Fagundes Ito, Marina Kiyomi Magalhães, Kelly Grace
Assunto:	Ácidos graxos Ômega-3 Piroptose Mamas - câncer DHA - ácido docosa-hexaenoico
Data de publicação:	2018
Editora:	Springer Nature
Referência:	PIZATO, Nathalia et al. Omega-3 docosahexaenoic acid induces pyroptosis cell death in triple-negative breast cancer cells. Scientific Reports, v. 8, art. n. 1952, 2018. DOI: 10.1038/s41598-018-20422-0. Disponível em: https://www.nature.com/articles/s41598-018-20422-0. Acesso em: 22 jan. 2018.
Abstract:	The implication of inflammation in pathophysiology of several type of cancers has been under intense investigation. Omega-3 fatty acids can modulate inflammation and present anticancer effects, promoting cancer cell death. Pyroptosis is an inflammation related cell death and so far, the function of docosahexaenoic acid (DHA) in pyroptosis cell death has not been described. This study investigated the role of DHA in triggering pyroptosis activation in breast cancer cells. MDA-MB-231 breast cancer cells were supplemented with DHA and inflammation cell death was analyzed. DHA-treated breast cancer cells triggered increased caspase-1and gasdermin D activation, enhanced IL-1β secretion, translocated HMGB1 towards the cytoplasm, and membrane pore formation when compared to untreated cells, suggesting DHA induces pyroptosis programmed cell death in breast cancer cells. Moreover, caspase-1 inhibitor (YVAD) could protect breast cancer cells from DHA-induced pyroptotic cell death. In addition, membrane pore formation showed to be a lysosomal damage and ROS formation-depended event in breast cancer cells. DHA triggered pyroptosis cell death in MDA-MB-231by activating several pyroptosis markers in these cells. This is the first study that shows the effect of DHA triggering pyroptosis programmed cell death in breast cancer cells and it could improve the understanding of the omega-3 supplementation during breast cancer treatment.
Licença:	Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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