| Campo DC | Valor | Idioma |
|---|
| dc.contributor.author | Graça, Grazielle Cardoso da | pt_BR |
| dc.contributor.author | Volpini, Angela Cristina | pt_BR |
| dc.contributor.author | Romero, Gustavo Adolfo Sierra | pt_BR |
| dc.contributor.author | Oliveira Neto, Manoel Paes de | pt_BR |
| dc.contributor.author | Hueb, Marcia | pt_BR |
| dc.contributor.author | Porrozzi, Renato | pt_BR |
| dc.contributor.author | Boité, Mariana Côrtes | pt_BR |
| dc.contributor.author | Cupolillo, Elisa | pt_BR |
| dc.date.accessioned | 2017-12-07T04:58:14Z | - |
| dc.date.available | 2017-12-07T04:58:14Z | - |
| dc.date.issued | 2012-08 | pt_BR |
| dc.identifier.citation | GRACA, Grazielle Cardoso da et al. Development and validation of PCR-based assays for diagnosis of American cutaneous leishmaniasis and identificatio nof the parasite species. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 107, n. 5, p. 664-674, ago. 2012. DOI: https://doi.org/10.1590/S0074-02762012000500014. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000500014&lng=en&nrm=iso. Acesso em: 04 dez. 2020. | pt_BR |
| dc.identifier.uri | http://repositorio.unb.br/handle/10482/28305 | - |
| dc.language.iso | en | pt_BR |
| dc.publisher | Instituto Oswaldo Cruz, Ministério da Saúde | pt_BR |
| dc.rights | Acesso Aberto | pt_BR |
| dc.title | Development and validation of PCR-based assays for diagnosis of American cutaneous leishmaniasis and identificatio nof the parasite species | pt_BR |
| dc.type | Artigo | pt_BR |
| dc.subject.keyword | Leishmaniose | pt_BR |
| dc.subject.keyword | Diagnóstico molecular | pt_BR |
| dc.subject.keyword | Identificação de espécies | pt_BR |
| dc.subject.keyword | Reação em cadeia de polimerase | pt_BR |
| dc.rights.license | Memórias do Instituto Oswaldo Cruz - All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License (CC BY NC 4.0). Fonte: https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000500014&lng=en&tlng=en. Acesso em: 04 dez. 2020. | - |
| dc.identifier.doi | https://dx.doi.org/10.1590/S0074-02762012000500014 | pt_BR |
| dc.description.abstract1 | In this study, PCR assays targeting different Leishmania heat-shock protein 70 gene (hsp70) regions, producing fragments ranging in size from 230-390 bp were developed and evaluated to determine their potential as a tool for the specific molecular diagnosis of cutaneous leishmaniasis (CL). A total of 70 Leishmania strains were analysed, including seven reference strains (RS) and 63 previously typed strains. Analysis of the RS indicated a specific region of 234 bp in the hsp70 gene as a valid target that was highly sensitive for detection of Leishmania species DNA with capacity of distinguishing all analyzed species, after polymerase chain reaction-restriction fragment length polymorfism (PCR-RFLP). This PCR assay was compared with other PCR targets used for the molecular diagnosis of leishmaniasis: hsp70 (1400-bp region), internal transcribed spacer (ITS)1 and glucose-6-phosphate dehydrogenase (G6pd). A good agreement among the methods was observed concerning the Leishmania species identification. Moreover, to evaluate the potential for molecular diagnosis, we compared the PCR targets hsp70-234 bp, ITS1, G6pd and mkDNA using a panel of 99 DNA samples from tissue fragments collected from patients with confirmed CL. Both PCR-hsp70-234 bp and PCR-ITS1 detected Leishmania DNA in more than 70% of the samples. However, using hsp70-234 bp PCR-RFLP, identification of all of the Leishmania species associated with CL in Brazil can be achieved employing a simpler and cheaper electrophoresis protocol. | - |
| dc.description.unidade | Faculdade de Medicina (FMD) | - |
| Aparece nas coleções: | Artigos publicados em periódicos e afins
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