Título:	Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex
Autor(es):	Soares, Marco Aurélio Araújo, Roberta Amália de Carvalho Marini, Marjorie Mendes Oliveira, Luciana Márcia de Lima, Leonardo Gomes de Alves, Viviane de Souza Felipe, Maria Sueli Soares Brígido, Marcelo de Macedo Soares, Célia Maria de Almeida Silveira, José Franco da Ruiz, Jerônimo Conceição Cisalpino, Patrícia Silva
Assunto:	Paracoccidioides Fungos América Latina Genoma
Data de publicação:	12-Mai-2015
Editora:	BioMed Central
Referência:	SOARES, Marco Aurélio et al. Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex. BMC Genomics, v. 16, Article 376, 12 mai. 2015. Disponível em: <https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-015-1564-7>. Acesso em: 27 jun. 2017. doi: https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-015-1564-7.
Abstract:	Background: Species from the Paracoccidioides complex are thermally dimorphic fungi and the causative agents of paracoccidioidomycosis, a deep fungal infection that is the most prevalent systemic mycosis in Latin America and represents the most important cause of death in immunocompetent individuals with systemic mycosis in Brazil. We previously described the identification of eight new families of DNA transposons in Paracoccidioides genomes. In this work, we aimed to identify potentially active retrotransposons in Paracoccidioides genomes. Results: We identified five different retrotransposon families (four LTR-like and one LINE-like element) in the genomes of three Paracoccidioides isolates. Retrotransposons were present in all of the genomes analyzed. P. brasiliensis and P. lutzii species harbored the same retrotransposon lineages but differed in their copy numbers. In the Pb01, Pb03 and Pb18 genomes, the number of LTR retrotransposons was higher than the number of LINE-like elements, and the LINE-like element RtPc5 was transcribed in Paracoccidioides lutzii (Pb01) but could not be detected in P. brasiliensis (Pb03 and Pb18) by semi-quantitative RT-PCR. Conclusion: Five new potentially active retrotransposons have been identified in the genomic assemblies of the Paracoccidioides species complex using a combined computational and experimental approach. The distribution across the two known species, P. brasiliensis and P. lutzii, and phylogenetics analysis indicate that these elements could have been acquired before speciation occurred. The presence of active retrotransposons in the genome may have implications regarding the evolution and genetic diversification of the Paracoccidioides genus.
Licença:	© 2015 Soares et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
DOI:	https://dx.doi.org/10.1186/s12864-015-1564-7
Aparece nas coleções:	Artigos publicados em periódicos e afins UnB - Professores Eméritos

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