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Título: Inhibition of feline leukemia virus replication in chronically infected cell line utilizing RNA interference
Autor(es): Ornelas, Sócrates Souza
Barra, Gustavo Barcelos
Kanzaki, Luis Isamu Barros
Assunto: Vírus da leucemia felina
RNA interferente
Gato - doenças
Data de publicação: 2012
Editora: Libertas Academica
Referência: Ornelas, Sócrates S.; BARRA, Gustavo B.; KANZAKI, Luis I.B. Inhibition of feline leukemia virus replication in chronically infected cell line utilizing RNA interference. Retrovirology: Research and Treatment, v. 4, p. 13-20, 2012. Disponível em: <http://www.la-press.com/inhibition-of-feline-leukemia-virus-replication-in-chronically-infecte-article-a3335>. Acesso em: 5 jun. 2013.
Resumo: Feline Leukemia virus (FeLV) is a pathogenic retrovirus endemic among domestic cats, remaining a serious disease since its discovery in 1964. RNA interference (RNAi) is a process in which double-stranded RNA induces the post-transcriptional sequence-specific degradation of homologous messenger RNA. At present, RNAi technology is regarded as a potential strategy for the treatment of various diseases as it can be used to inhibit the expression of desired peptides/proteins. This study aimed to apply RNAi technology to inhibit the replication of FeLV. We examined the effect of vector-mediated transfer and expression of FeLV specific short hairpin RNA (shRNA) against p27 protein expression and replication of FeLV in a feline T-cell line chronically infected with FeLV (3201-EECC). Three shRNA homologous to the FeLV gag gene were synthesized, cloned and transfected into a feline fibroblastic cell line (CrFK) expressing FeLV which efficiently reduced FeLV p27 protein expression, consequently decreasing and inhibiting the viral replication in a chronically FeLV infected feline T-cell line (3201-EECC). The expression of shRNA against FeLV gag gene showed markedly lower p27 levels and viral replication in both cell lines, 3201-EECC and CrFK. These results provide useful information to pave the road for the development of a gene therapy strategy to control FeLV and related pathogenic retrovirus infections in the future.
Licença: Retrovirology: Research and Treatment - This is an open access article. Unrestricted non-commercial use is permitted provided the original work is properly cited. Fonte: http://www.la-press.com/inhibition-of-feline-leukemia-virus-replication-in-chronically-infecte-article-a3335. Acesso em: 5 jun. 2013.
DOI: https://dx.doi.org/10.4137/RRT.S8401
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